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Pharmacology of Hormonal Polypeptides and Proteins pp 317–335Cite as

Insulin Secretion “In Vitro”: Comparative Aspects and Comparisons with Studies on Release of Insulin “In Vivo”

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 2))

Abstract

In the preceding papers many studies were reported regarding the release of insulin “in vivo” in animals and men, mainly under the aspects of gastrointestinal control of insulin secretion. I will take a slightly different position. I shall describe experiments performed in collaboration with Drs. Telib, Ammon, Hildebrandt, Raptis, Schröder, Veboah, Schwarz and Melani, in which

  1. 1)

    insulin secretion “in vitro” was studied in the presence of different sugars and amino acids, polypeptide hormones of gastrointestinal origin and sulfonylureas of different β-cytotropic activities,

  2. 2)

    the response of the pancreatic tissue of various species of animals including amphibians was examined

  3. 3)

    the relation between the extractable pancreatic insulin content and the amount of insulin released following stimulation was investigated as well as the conceivable incorporation of glucose and amino acids into the insulin molecule.

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References

  • Biermann E. L., E. M. Bakar, J. C. Plough and W. H. Hall: Metabolism of d-ribose in diabetes mellitus. Diabetes 8, 455, (1959)

    Google Scholar 

  • Candela J. L. R., R. R. Candela, D. Martin and T. Hernandez and Castilla Costazar: Insulin secretion “in vitro” (prelim.comm.), Proc.4th Congr.I. D. F. Geneva (1961) p.629

    Google Scholar 

  • Candela J. L. R., R. R. Candela, D. Martin and T. Hernandez and Castilla Costazar and H. G. Coore: Insulin secretion “in vitro” in: Handbuch des Diabetes mellitus, Pathophysiologie und Klinik, Ed. E. F. Pfeiffer, Lehmanns Verlag, München (in press)

    Google Scholar 

  • Coore G. and Rändle P. J.: Regulation of insulin secretion studied with pieces of rabbit pancreas. Biochem. J. 93, 65, (1964)

    Google Scholar 

  • Davoren P. R.: The isolation of insulin from a single rat pancreas. Biochem.Biophys. Acta 63, 150, (1962)

    Article  PubMed  CAS  Google Scholar 

  • Delaney J. P. and E. Grim: Influence of hormones and drugs on canine pancreatic blood flow. Am.J.Physiol., 211, 1398–1402, 1966

    PubMed  CAS  Google Scholar 

  • Ditschuneit H., J. P. Faulhaber und E. F. Pfeiffer: Verbesserung der Methode zur Bestimmung von Insulin im Blut mit Hilfe radioaktiver 14J-C-Glukose und dem epididymalen Rattenfettgewebe. Atompraxis 8, 172 (1962)

    CAS  Google Scholar 

  • Floyd J. C., S. S. Fajans, J. W. Conn, R. F. Knopf and J. Rull: Insulin secretion in response to protein ingestion D. Clin.Invest. 45, 1479 (1966)

    Article  CAS  Google Scholar 

  • Grodsky G. M. and L. L. Bennett: Insulin secretion from the isolated pancreas in absence of insulino-genesis: effect of glucose. Proc.Soc.Exper.Biol.and Med. 114, 769 (1963)

    Article  CAS  Google Scholar 

  • Grodsky G. M. and L. L. Bennett and D. F. Smith: Effect of Carbohydrates on secretion of insulin from isolated rat pancreas. Amer.J.Physiol. 205, 638 (1963)

    PubMed  CAS  Google Scholar 

  • Grodsky G. M., L. L. Bennett, D. F. Smith and F. C. Schmid: Effect of pulse administration of glucose or glucagon on insulin secretion in vitro. Metabolism 16, 222 (1967)

    Article  PubMed  CAS  Google Scholar 

  • Hanke W. and K.Bergerhoff: (unpubl.)

    Google Scholar 

  • Hildebrand H. E.: Contribution to discussion. Communication to Stop-Press-Session, 6th Congr. IDF Stockholm, Sweden, Aug. 1967

    Google Scholar 

  • Malaisse W., F. Malaisse-Lagae and P. H. Wright: A new method for the measurement in vitro of pancreatic insulin secretion. Endocrinology 80, 99 (1967)

    Article  PubMed  CAS  Google Scholar 

  • Martin D. B., A. E. Renold and Y. M. Dagenais: An assay for insulin-like-activity using rat adipose tissue. Lancet II, 76 (1958)

    Article  Google Scholar 

  • Meier J. M., J. Ammon, U. Gröschel-Stewart, F. Melani, J. E. Yeboah und E. F. Pfeiffer: Eine einfache, schnelle Methode zur Bestimmung von Insulin in kleinsten Gewebsmengen (bis 10 mg), 13.Symp.Dtsch.Ges.Endocrin. Würzburg 2.-4.-3.-1967, Abstr.Nr.57 (in press)

    Google Scholar 

  • Melani F., J. Lawecki, K. M. Bartelt und E. F. Pfeiffer: Insulinspiegel bei Stoffwechselgesunden, Fettsüchtigen und Diabetikern nach intravenöser Gabe von Glukose, Tolbutamid und Glukagon. Abstr.Nr.115, 2.Tg.Europ.Ges.f.Diabetol. 1966, Diabetologia (in press)

    Google Scholar 

  • Moskalewski S.: Isolation and culture of the islets of Langerhans of the guinea pig. Gen.and Comp.Endoc. 5, 342 (1965)

    Article  Google Scholar 

  • Pfeiffer E. F.: Die Immunologie des Insulins. Verh.Dtsch.Ges.Inn.Med. 72, 811 (1966)

    Google Scholar 

  • Pfeiffer E. F.: Introduction to Panel Discussion “Intestinal Function in Relation to Insulin Secretion”. 6th Intern.Congr.I. D. F. Stockholm 1967

    Google Scholar 

  • Pfeiffer E. F. und M.Telib: 1967 (in preparation)

    Google Scholar 

  • Pfeiffer E. F. und M. Telib, H. Steigerwald, W. Sandritter, A. Bänder, A. Mager, U. Becker und K. Retiene: Vergleichende Untersuchungen von Morphologie und Hormongehalt des Kälberpancreas nach Sulfonylharnstoffen (D 860). Dtsch.Med.Wschr. 82, 1568 (1957)

    Article  PubMed  CAS  Google Scholar 

  • Pfeiffer E. F. und M. Telib, H. Steigerwald, W. Sandritter, A. Bänder, A. Mager, U. Becker und K. Retiene, M. Telib, J. Ammon, F. Melani und H. Ditschuneit: Direkte Stimulierung der Insulinsekretion in vitro durch Sekretin. Dtsch. Med. Wschr. 90, 1663 (1965)

    Article  PubMed  CAS  Google Scholar 

  • Pfeiffer E. F. und M. Telib, H. Steigerwald, W. Sandritter, A. Bänder, A. Mager, U. Becker und K. Retiene, M. Telib, J. Ammon, F. Melani und H. Ditschuneit, J. E. Yeboah, M. Telib, J. Ammon, H. E. Hildebrandt, Becker und Meier: 1967 (in preparation)

    Google Scholar 

  • Pozza G., G. Galansino, H. Hoffield and P. Foa: Stimulation of insulin output by monosaccharides and monosaccharides derivates. Amer.J.Physiol. 192, 497 (1958)

    PubMed  CAS  Google Scholar 

  • Raptis S., K. E. Schröder, F. Melani und E. F. Pfeiffer: Die Beeinflussung der Insulinsekretion durch Secretin in vivo. Panel discussion “Intestinal Function in Relation to Insulin Secretion”. — 6th Congr.I. D. F. Stockholm, Aug. 1967

    Google Scholar 

  • Schöffling K.: Der Insulinstoffwechsel des pancreaslosen Hundes. — 12.Symp.Dtsch.Ges.Endokrinologie, Wiesbaden (1966)

    Google Scholar 

  • Schröder K. E., S. Raptis, M. Telib und E. F. Pfeiffer: Intestinal Hormones effecting insulin secretion in vitro: Secretin, Pancreocymin and Gastrin. Panel Discussion “Intestinal Function in Relation to Insulin Secretion”. — 6th Congr. I. D. F. Stockholm, Aug.1967

    Google Scholar 

  • Schwarz H., J. Ammon, J. E. Yeboah, H. E. Hildebrandt und E. F. Pfeiffer: Förderung der Insulinsekretion in vitro durch ein neues, hochwirksames Antidiabeticum. Diabetologia 1967 (in press)

    Google Scholar 

  • Telib M.: Der Einfluß von Monosacchariden und Hormonen auf die Insulinsekretion verschiedener Wirbeltiere. Inaug.Diss. Frankfurt (1967)

    Google Scholar 

  • Vecchio D., A. Luyckx, C. R. Zahnd and A. E. Renold: Insulin release induced by glucagon in organ cultures of fetal rat pancreas. Metabolism 15, 577 (1966)

    Article  PubMed  CAS  Google Scholar 

  • Williams R. H.: Secretion, Tates and action of insulin and related products. Diabetes 15, 623 (1966)

    PubMed  CAS  Google Scholar 

  • Yeboah J. E.: Insulin secretion in vitro: Stimulation der Insulinsekretion in vitro durch einzelne Aminosäuren. Inaug.Diss. Ulm (1967)

    Google Scholar 

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Author information

Authors and Affiliations

  1. Dept.of Clinical Endocrinology and Metabolism, Center of Medicine, University of Ulm, Donau, Germany

    Ernst F. Pfeiffer

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  1. Ernst F. Pfeiffer
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Editors and Affiliations

  1. Department of Biochemical Pharmacology, School of Pharmacy, State University of New York, Buffalo, New York, USA

    Nathan Back

  2. Institute of Pharmacology, University of Milan, Milan, Italy

    Luciano Martini

  3. Institute of Pharmacology, University of Cagliari, Cagliari, Italy

    Rodolfo Paoletti (Chairman) (Chairman)

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Pfeiffer, E.F. (1968). Insulin Secretion “In Vitro”: Comparative Aspects and Comparisons with Studies on Release of Insulin “In Vivo”. In: Back, N., Martini, L., Paoletti, R. (eds) Pharmacology of Hormonal Polypeptides and Proteins. Advances in Experimental Medicine and Biology, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-4612-5_39

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  • DOI: https://doi.org/10.1007/978-1-4614-4612-5_39

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-2726-2

  • Online ISBN: 978-1-4614-4612-5

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