Advertisement

Neurophysin, Oxytocin and Vasopressin in Neurosecretory Granules and in Crystalline Complexes

  • D. B. Hope
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 2)

Abstract

Sometime ago it was noticed in this laboratory that neurophysin was composed of several proteins separable by electrophoresis in starch gels.1 Previously bovine neurophysin had been considered to be a homogeneous protein2 which could bind both of the pituitary polypeptide hormones, oxytocin and vasopressin. A complex containing all three constituents isolated by van Dyke and his coworkers3 possessed oxytocic and pressor activities in a ratio of 1:1. Landgrebe, Ketterer and Waring4 pointed out that the biological activities of the van Dyke protein corresponded to a complex of a Mole of oxytocin and of vasopressin bound per Mole of protein. The fact that neurophysin was a mixture of proteins was of interest because of evidence for the independent release of oxytocin and vasopressin 5,6. Of this interpretation were to prove correct then the all-or-none nature of neurone activation requires that oxytocin cannot be stored in the same granule as vasopressin. No histological methods for distinguishing oxytocin from vasopressin in neurones exists. A biochemical approach would be the isolation of two kinds of neurosecretory granules, the one containing only oxytocin and the other containing only vasopressin.

Keywords

Amino Acid Analysis Crystalline Complex Granule Protein Polypeptide Hormone Acetone Powder 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Hope, D.B., Schacter, B.A. & Frankland, B.T.B. (1964). Biochem. J. 93, 7P.Google Scholar
  2. 2.
    Acher, R., Chauvet, J. & Olivry, G. (1956). Biochim. Biophys. Acta, 22, 421.PubMedCrossRefGoogle Scholar
  3. 3.
    van Dyke, H.B., Chow, B.F., Greep, R.O. & Rothen, A. (1942). J, Pharmacol., 74, 190.Google Scholar
  4. 4.
    Landgrebe, F.W., Ketterer, B. de Waring, H. (1955). In: The Hormones, Vol. 111 Eds. Pincus, G. & Thimann, K.V. Academic Press, New York pp 389–431.Google Scholar
  5. 5.
    Ginsburg, M. & Smith, M.W. (1959). Brit. J. Pharmacol. 14, 327.PubMedGoogle Scholar
  6. 6.
    Gaitan, E., Cobo, E. & Mizrachi, M. (1964). J. clin. Invest. 43, 2310.PubMedCrossRefGoogle Scholar
  7. 7.
    La Bella, F.S., Reiffenstein, R.J. & Beaulieu, G. (1963). Arch. Biochem. Biophys. 100, 399.CrossRefGoogle Scholar
  8. 8.
    Barer, R., Heller, H. & Lederis, K.(1963). Proc. Roy. Soc B., 158, 388.CrossRefGoogle Scholar
  9. 9.
    Hope, D.B. (1964). In: Oxytocin Vasopressin and their structural analogues, p. 105. Ed. by Rudinger, J. London: Pergamon Press.Google Scholar
  10. 10.
    Chauvet, J., Lenci, M.-T. & Acher, R.(1960). Biochim. Biophys. Acta, 38, 266.PubMedCrossRefGoogle Scholar
  11. 11.
    Smithies, O. (1955). Biochem. J. 61, 629.PubMedGoogle Scholar
  12. 12.
    Poulik, M.D. (1957). Nature, Lond. l80, 1477.CrossRefGoogle Scholar
  13. 13.
    Frankland, B.T.B., Hollenberg, M.D., Hope, D.B. & Schacter, B.A. (1966). Brit. J. Pharmacol. 26, 502.PubMedGoogle Scholar
  14. 14.
    Block, R.J. & van Dyke, H.B. (1950). Nature, Lond. 165, 975.CrossRefGoogle Scholar
  15. 15.
    Block, R.J. & van Dyke, H.B. (1952). Arch. Biochem. Biophys. 36, 1.CrossRefGoogle Scholar
  16. 16.
    Hope, D.B. & Hollenberg, M.D. (1966). Biochem. J. 99, 5P.Google Scholar
  17. 17.
    Hollenberg, M.D. & Hope, D.B. (1967). Biochem. J. 104, 122.PubMedGoogle Scholar
  18. 18.
    Ferguson, K.A. & Wallace, A.L.C. (1961). Nature, Lond. 190. 629.CrossRefGoogle Scholar
  19. 19.
    Dean, C.R. & Hope, D.B. (1966). Biochem. J. 101, 17P.Google Scholar
  20. 20.
    Dean, C.R. & Hope, D.B. (1967). Biochem. J. In Press.Google Scholar
  21. 21.
    Adams, E. & Smith, E.L. (1951). J. biol. Chem. 191, 651.PubMedGoogle Scholar
  22. 22.
    Raaachandran, L.K. & Winnick, T. (1957). Biochim. biophys .Acta, 23, 533.CrossRefGoogle Scholar
  23. 24.
    Dean, C.R., Hollenberg, M.D. & Hope, D.B. (1967). Biochem. J. 104, 8C.Google Scholar
  24. 25.
    Winnick, T., Winnick, R.E., Acher, R. & Fromageot, C. (1955). Biochim. biophys. Acta, 18, 488.PubMedCrossRefGoogle Scholar
  25. 26.
    Hollenberg, M.D. & Hope, D.B. (1966). J. Physiol. 185, 51P.Google Scholar
  26. 27.
    Webb, E.C. (1964). Nature, Lond., 203, 821.Google Scholar
  27. 28.
    Yphantis, D.A. (1960). Ann. N.Y. Acad. Sci., 88, 586.CrossRefGoogle Scholar
  28. 29.
    Low, B.R. & Edsall, J.T. (1956). In: Currents in Biochemical Research, p. 378. Ed. Green, D.E. New York: Interscience Publishers Inc.Google Scholar
  29. 30.
    Szent-Györgyi, A. & Cohen, C. (1957). Science, 126, 697.PubMedCrossRefGoogle Scholar
  30. 31.
    Perutz, M.F. (1962). Nature, Lond., 194, 914.CrossRefGoogle Scholar
  31. 32.
    Breslow, E. & Abrash, L. (1966). Proc. Nat. Acad. Sci. Wash. 56, 640.CrossRefGoogle Scholar
  32. 33.
    Acher, R., Chauvet, J. & Lenci, M.-T. (1960). Biochim. biophys. Acta, 38, 344.PubMedCrossRefGoogle Scholar
  33. 34.
    Acher, R., Chauvet, J., Lenci, M.-T., Morel, F. & Maetz, J. (1960). Biochim. biophys. Acta, 42, 379.PubMedCrossRefGoogle Scholar
  34. 35.
    Stouffer, J.E., Hope, D.B. & du Vigneaud, V. (1962). In: Perspectives in Biology pp 75–80. Ed. by Cori, C.F., Foglia, V.G., Leloir, L.F. & Ochoa, S. The Netherlands: Elsevier Publishing Co.Google Scholar
  35. 36.
    Hollenberg, M.D. & Hope, D.B. (1967). Biochem. J. In Press.Google Scholar
  36. 37.
    Walsh, K.A., Ericsson, L.H. & Neurath, H. (1966). Proc. Nat. Acad. Sci., Wash. 56, 1339.CrossRefGoogle Scholar
  37. 38.
    Blaschko, H., Comline, R.S., Schneider, F.H., Silver, M. & Smith, A.D. (1967). Nature, Lond. 215, 58.CrossRefGoogle Scholar
  38. 39.
    Rennels, M.L. (1966). Endocrinology, 78, 659.PubMedCrossRefGoogle Scholar
  39. 40.
    Friesen, H.G. & Astwood, E.B. (1967). Endocrinology, 80, 278.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1968

Authors and Affiliations

  • D. B. Hope
    • 1
  1. 1.Department of PharmacologyUniversity of OxfordUK

Personalised recommendations