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Isolation of Collagen from Cortical Bovine Bone for Preparation of Porous Collagen Sponges

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Mechanics of Biological Systems and Materials, Volume 5

Abstract

Fabrication of collagen sponges from type I collagen isolated from cortical bovine femur bone is reported. Collagen was extracted with 0.1 M EDTA by refreshing the solution every 24 h for 9 days. Sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE) demonstrated the isolation of intact pure type I collagen. The sponges were fabricated by freeze-drying and heat-dehydration cross-linking of collagen solution. The resulting sponges showed fibrils arranged in a highly porous network. These sponges can be used for tissue engineering as well as for bone-like composite fabrication.

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Acknowledgements

We thank Ryan Anderson of CalIT2, UCSD, for help in SEM imaging. Ana Castro acknowledges the University of California Institute for Mexico and the United States (UC MEXUS-CONACyT). This work has been founded by the Ceramics Program (Grant# DMR1006931).

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Authors and Affiliations

  1. Materials Science and Engineering Program, University of California, San Diego, 9500, Gilman Dr., La Jolla, CA, 92093, USA

    Ana B. Castro-Ceseña, Ekaterina E. Novitskaya & Joanna McKittrick

  2. Department of Bioengineering, University of California, San Diego, 9500, Gilman Dr., La Jolla, CA, 92093, USA

    Ameya Phadke & Shyni Varghese

  3. Department of Mechanical and Aerospace Engineering, University of California, San Diego, 9500, Gilman Dr., La Jolla, CA, 92093, USA

    Joanna McKittrick

Authors
  1. Ana B. Castro-Ceseña
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  2. Ekaterina E. Novitskaya
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  3. Ameya Phadke
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  4. Shyni Varghese
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  5. Joanna McKittrick
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Corresponding author

Correspondence to Ana B. Castro-Ceseña .

Editor information

Editors and Affiliations

  1. Auburn University, Alabama, Auburn, 36849-5341, USA

    Barton C. Prorok

  2. McGill University, Montreal, H4B 2R3, Canada

    François Barthelat

  3. State University of New York, State University of New York, Stony Brook, 11794, USA

    Chad S. Korach

  4. Rice University, Houston, 77251-1892, USA

    K. Jane Grande-Allen

  5. Auburn University, Auburn, 36849-5127, USA

    Elizabeth Lipke

  6. University of Connecticut, Storrs, 06269-3088, USA

    George Lykofatitits

  7. Purdue University, West Lafayette, 47907-2051, USA

    Pablo Zavattieri

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© 2013 The Society for Experimental Mechanics, Inc.

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Castro-Ceseña, A.B., Novitskaya, E.E., Phadke, A., Varghese, S., McKittrick, J. (2013). Isolation of Collagen from Cortical Bovine Bone for Preparation of Porous Collagen Sponges. In: Prorok, B., et al. Mechanics of Biological Systems and Materials, Volume 5. Conference Proceedings of the Society for Experimental Mechanics Series. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-4427-5_11

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  • DOI: https://doi.org/10.1007/978-1-4614-4427-5_11

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-4426-8

  • Online ISBN: 978-1-4614-4427-5

  • eBook Packages: EngineeringEngineering (R0)

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