Bioaugmentation for Aerobic Degradation of CIS-1,2-Dichloroethene
Polaromonas sp. strain JS666 is the first isolate capable of using cis-dichloroethene (cis-DCE) as its sole carbon and energy source under aerobic conditions (Coleman et al., 2002a). It is a promising candidate for bioaugmentation at cis-DCE-contaminated sites where cis-DCE has migrated downgradient into an aerobic zone. Addition of the strain can circumvent the problems associated with cometabolic oxidation as a bioremediation strategy because it catalyzes rapid degradation without the addition of a cosubstrate, and the requirements for oxygen are much lower than for cometabolic transformations. The metabolic capabilities of JS666, development of a molecular probe for process monitoring, microcosm assessment of site suitability, and the preliminary results of a field-scale study are discussed in this chapter.
KeywordsVinyl Chloride Injection Well Minimal Salt Medium Reductive Dechlorination Savannah River Site
This research was supported in part by contracts with the Environmental Security Technology Certification Program (ESTCP) and the SERDP. L.K. Jennings and C. G. S. Giddings were recipients of National Science Foundation (NSF) Graduate Research Fellowships. The authors thank F. Liu, E. Wood, and S. Nishino for sharing unpublished data; and D. Major, C. Aziz, and M. Watling of Geosyntec Consultants, Inc., who conducted the bioaugmentation field study at St. Julien’s Creek Annex, Virginia.
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