Functional Analysis of HMG-CoA Reductase and Oxidosqualene Cyclases in Arabidopsis
In plants, more than 200 triterpenes with various carbon skeletons have been found, of which some triterpenes are biologically active. Here we show recent detailed analyses on the biosynthetic genes and the mechanism of triterpene biosynthesis characteristic of higher plants. The studies presented here employed molecular genetic tools and organic chemistry methods to focus on the function of HMG-CoA reductase, the triterpene biosynthesis rate-limiting enzyme, and oxidosqualene cyclase, the enzyme catalyzing the formation of the cyclic triterpene skeletons.
KeywordsArabidopsis Lanosterol Mevalonate Mutant NMR Oxidosqualene cyclase Sterol Triterpenoid
This work was supported in part by Grants-in-Aid for Scientific Research and Bilateral Program from JSPS, Japan, and Strategic International Cooperative Program from JST, Japan.
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