Plasmon Biophotonic Arrays for Multi-analyte Biosensing in Complex Media
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The design of plasmon-based sensors for analysis of complex media such as serum is sensitive to the effects of non-specific binding. A simple analysis is presented to provide insight into the orders of magnitude involved in the kinetics of the problem and how label-free Immuno-kinetic assays may compensate for these effects. We then consider some specific challenges including sensitivity and non-specific binding discussed in terms of the kinetic and thermodynamic parameters of the protein–protein interactions which define the extent of fouling of the target sensor surface. Nanoparticle plasmon arrays have some fundamental advantages in multi-analyte sensing in complex media such as blood and the advantages of multiple measurements are considered in the context of a global mechanistic, kinetic analysis to profile the complex medium composition.
KeywordsSurface Plasmon Resonance Human Serum Albumin Localise Surface Plasmon Resonance Sensor Surface Analyte Molecule
The work was supported by the RCUK, Basic Technology Grant, EP/C52389X/1 and the Royal Society.
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