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Spectroscopic Characterization of a New Antibacterial Material for Sensing Applications

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Sensors

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 162))

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Abstract

A novel antibacterial system based on immobilization of glucose oxidase enzyme (GOx) in a poly(vinyl alcol) (PVA) film was proposed. The GOx/PVA composite material has been extensively characterized by UV-vis and X-ray photoelectron (XPS) spectroscopy to verify the preservation of the enzyme structural integrity and of the enzymatic activity in PVA membrane. Moreover, XPS characterization was used to analyze the chemistry of GOx/PVA composite film. The antibacterial lysozyme-like activity of GOx/PVA was evaluated by a standard assay on Petri dishes employing Micrococcus luteus cell walls. Thus the findings from this study have implications for future investigations related to employment of GOx/PVA as a compound of pharmaceutical and technological interest.

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Acknowledgments

This work was supported by Fondazione Cassa di Risparmio di Puglia (Viale della Repubblica 111, Bari – Italy) regarding the progect “Sviluppo e validazione di metodi basati sull’utilizzo di sensori elettrochimici micro e nano strutturati per la determinazione di analiti di interesse ambiantale in matrici reali”.

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Authors and Affiliations

  1. Lab. Chimica Analitica, Di.S.Te.B.A., Università del Salento, Via Monteroni, Lecce, 73100, Italy

    D. Chirizzi, M. R. Guascito & C. Malitesta

  2. Istituto per l’Ambiente Marino Costiero CNR U.O.S., Via Roma 3, Taranto, 74100, Italy

    L. Stabili

Authors
  1. D. Chirizzi
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  2. M. R. Guascito
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  3. C. Malitesta
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  4. L. Stabili
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Corresponding author

Correspondence to D. Chirizzi .

Editor information

Editors and Affiliations

  1. Institute of Applied Physics IFAC-CNR, Firenze, Italy

    Francesco Baldini

  2. University of Rome Tor Vergata, Rome, Italy

    Arnaldo D’Amico

  3. University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  4. Inst for Microelectronics & Microsystems IMM-CNR, Lecce, Lecce, Italy

    Pietro Siciliano

  5. Department of Chemistry, Univ. of Modena and Reggio Emilia, Modena, Italy

    Renato Seeber

  6. Inst for Microelectronics & Microsystems IMM-CNR, Napoli, Napoli, Italy

    Luca De Stefano

  7. Institute of Biophysics, IBF-CNR, Pisa, Pisa, Italy

    Ranieri Bizzarri

  8. DIEEI - Engineering Faculty, University of Catania, Catania, Italy

    Bruno Andò

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Chirizzi, D., Guascito, M.R., Malitesta, C., Stabili, L. (2014). Spectroscopic Characterization of a New Antibacterial Material for Sensing Applications. In: Baldini, F., et al. Sensors. Lecture Notes in Electrical Engineering, vol 162. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3860-1_28

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  • DOI: https://doi.org/10.1007/978-1-4614-3860-1_28

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-3859-5

  • Online ISBN: 978-1-4614-3860-1

  • eBook Packages: EngineeringEngineering (R0)

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