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Coupling Nanotechnology to Optical Affinity Sensing: The Case of Surface Plasmon Resonance Imaging for DNA Detection

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Sensors

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 162))

Abstract

Surface Plasmon Resonance imaging (SPRi) is an advanced optical transducer for the DNA affinity biosensors. SPRi signal enhancement of a DNA biosensor is a goal in developing innovative devices, especially for clinical applications. Nanoparticles (NPs) play a forefront role in signal enhancement in biosensor-based analysis, using different transduction principles. This work focused on the use of NPs for gold chip surface nanostructuring with the aim to study influence of this modification on DNA-based sensing using SPRi transduction. In particular gold NPs of different size and materials were immobilized through dithiol layer on gold biochip surface and further functionalized with thiolated DNA probe. Plasmon curves were studied for assessing the presence of the nanostructure and the influence of the nanostructure in SPRi signal was evaluated for each structure in hybridization with complementary oligonucleotide.

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References

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Authors and Affiliations

  1. Dipartimento di Chimica “Ugo Schiff” and CSGI, Università degli Studi di Firenze, Via della Lastruccia 3, Sesto Fiorentino, FI, 50019, Italy

    M. L. Ermini, S. Mariani, F. Bellissima, S. Scarano, M. Bonini & M. Minunni

Authors
  1. M. L. Ermini
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  2. S. Mariani
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  3. F. Bellissima
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  4. S. Scarano
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  5. M. Bonini
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  6. M. Minunni
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Corresponding author

Correspondence to M. Minunni .

Editor information

Editors and Affiliations

  1. Institute of Applied Physics IFAC-CNR, Firenze, Italy

    Francesco Baldini

  2. University of Rome Tor Vergata, Rome, Italy

    Arnaldo D’Amico

  3. University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  4. Inst for Microelectronics & Microsystems IMM-CNR, Lecce, Lecce, Italy

    Pietro Siciliano

  5. Department of Chemistry, Univ. of Modena and Reggio Emilia, Modena, Italy

    Renato Seeber

  6. Inst for Microelectronics & Microsystems IMM-CNR, Napoli, Napoli, Italy

    Luca De Stefano

  7. Institute of Biophysics, IBF-CNR, Pisa, Pisa, Italy

    Ranieri Bizzarri

  8. DIEEI - Engineering Faculty, University of Catania, Catania, Italy

    Bruno Andò

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Ermini, M.L., Mariani, S., Bellissima, F., Scarano, S., Bonini, M., Minunni, M. (2014). Coupling Nanotechnology to Optical Affinity Sensing: The Case of Surface Plasmon Resonance Imaging for DNA Detection. In: Baldini, F., et al. Sensors. Lecture Notes in Electrical Engineering, vol 162. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3860-1_17

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  • DOI: https://doi.org/10.1007/978-1-4614-3860-1_17

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-3859-5

  • Online ISBN: 978-1-4614-3860-1

  • eBook Packages: EngineeringEngineering (R0)

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