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Sensors pp 95–98Cite as

Hepcidin Detection by Affinity Based Sensing: A Possible Application in Clinical and Anti-doping Analysis

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Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 162))

Abstract

The human Hepcidin-25 is a cysteine-rich proteinic hormone able to play a key role in systemic iron homeostasis. Indeed it can be associated with inflammatory events and iron dysfunctions but it may be even used as a new bio-marker to estimate indirectly erythropoietin blood doping. Affinity-based biosensors (ABBs) could be applied to hepcidin detection. In this study we adopted optical sensor system for label-free detection, based on Surface Plasmon Resonance (SPR), using as bioreceptor an anti human hepcidin-25 IgG. Limitations, advantages and potentials of this system are briefly discussed in light of behaviours and analytical performances detected.

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References

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Acknowledgments

 Maria Minunni would like to thank the World Antidoping Agency (WADA), for funding within the “Scientific Research Grant-2010”, “Detection of Hepcidin As A New Biomarker of Erythropoiesis Stimulators Abuse: A Pilot Study”. We thank Dr. A. M. Bossi (University of Verona, Italy) and I. De Domenico (University of Utah, Internal Medicine Department, Hematology Division) for fruitful scientific discussions.

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Authors and Affiliations

  1. Dipartimento di Chimica, Università di Firenze, via della Lastruccia 3, Firenze, 5019, Italy

    Simona Scarano, Ambra Vestri, Maria Laura Ermini & Maria Minunni

  2. Università degli Studi di Verona, Ca’ Vignal 1, Strada Le Grazie 15, Verona, 37134, Italy

    Ambra Vestri

Authors
  1. Simona Scarano
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  2. Ambra Vestri
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  3. Maria Laura Ermini
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  4. Maria Minunni
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Corresponding author

Correspondence to Maria Minunni .

Editor information

Editors and Affiliations

  1. Institute of Applied Physics IFAC-CNR, Firenze, Italy

    Francesco Baldini

  2. University of Rome Tor Vergata, Rome, Italy

    Arnaldo D’Amico

  3. University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  4. Inst for Microelectronics & Microsystems IMM-CNR, Lecce, Lecce, Italy

    Pietro Siciliano

  5. Department of Chemistry, Univ. of Modena and Reggio Emilia, Modena, Italy

    Renato Seeber

  6. Inst for Microelectronics & Microsystems IMM-CNR, Napoli, Napoli, Italy

    Luca De Stefano

  7. Institute of Biophysics, IBF-CNR, Pisa, Pisa, Italy

    Ranieri Bizzarri

  8. DIEEI - Engineering Faculty, University of Catania, Catania, Italy

    Bruno Andò

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© 2014 Springer Science+Business Media New York

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Cite this paper

Scarano, S., Vestri, A., Ermini, M.L., Minunni, M. (2014). Hepcidin Detection by Affinity Based Sensing: A Possible Application in Clinical and Anti-doping Analysis. In: Baldini, F., et al. Sensors. Lecture Notes in Electrical Engineering, vol 162. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3860-1_15

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  • DOI: https://doi.org/10.1007/978-1-4614-3860-1_15

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-3859-5

  • Online ISBN: 978-1-4614-3860-1

  • eBook Packages: EngineeringEngineering (R0)

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