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Experimental and Clinical Metastasis pp 279–284Cite as

Diagnostic Immunohistochemistry in Tumor Metastasis

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Abstract

Immunohistochemistry (IHC) refers to the process of localizing antigens (e.g. proteins) in cells of a tissue section using antibodies that bind specifically to these antigens in bisological tissues Ramos-Vara (Vet Pathol 42:405–426, 2005). Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors and in metastasis Webster et al. (J Histochem Cytochem 57:753–761, 2009).

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References

  • Bibeau F et al (2006) Assessment of epidermal growth factor receptor (EGFR) expression in primary colorectal carcinomas and their related metastases on tissue sections and tissue microarray. Virchows Arch 449:281–287

    Article  PubMed  CAS  Google Scholar 

  • Buchwalow IB, Böcker W (ed) (2010) Antibodies for immunohistochemistry. SpringerLink, p 153

    Google Scholar 

  • Colcher D, Hand PH, Nuti M, Schlom J (1981) A spectrum of monoclonal antibodies reactive with human mammary tumor cells. Proc Natl Acad Sci U S A 78:3199–3203

    Article  PubMed  CAS  Google Scholar 

  • Dällenbach P, Bonnefoi H, Pelte MF, Vlastos G (2006) Yolk sac tumours of the ovary: an update. Eur J Surg Oncol 32:1063–1075

    Article  PubMed  Google Scholar 

  • Fernando SS, Johnson S, Bate J (1994) Immunohistochemical analysis of cutaneous malignant melanoma: comparison of S-100 protein, HMB-45 monoclonal antibody and NKI/C3 monoclonal antibody. Pathology 26:16–19

    Article  PubMed  CAS  Google Scholar 

  • Gleissner B et al (2005) CD10- pre-B acute lymphoblastic leukemia (ALL) is a distinct high-risk subgroup of adult ALL associated with a high frequency of MLL aberrations: results of the German Multicenter Trials for Adult ALL (GMALL). Blood 106:4054–4056

    Article  PubMed  CAS  Google Scholar 

  • Gold JS, Dematteo RP (2006) Combined surgical and molecular therapy: the gastrointestinal stromal tumor model. Ann Surg 244:176–184

    Article  PubMed  Google Scholar 

  • Guzman G et al (2005) p53, Ki-67, and serum alpha feto-protein as predictors of hepatocellular carcinoma recurrence in liver transplant patients. Mod Pathol 18:1498–1503

    Article  PubMed  CAS  Google Scholar 

  • Hans CP et al (2004) Confirmation of the molecular classification of diffuse large B-cell lymphoma by immunohistochemistry using a tissue microarray. Blood 103:275–282

    Article  PubMed  CAS  Google Scholar 

  • Hansen HJ et al (1974) Carcinoembryonic antigen (CEA) assay. A laboratory adjunct in the diagnosis and management of cancer. Hum Pathol 5:139–147

    Article  PubMed  CAS  Google Scholar 

  • Harari PM (2004) Epidermal growth factor receptor inhibition strategies in oncology. Endocr Relat Cancer 11:689–708

    Article  PubMed  CAS  Google Scholar 

  • Ikeda S et al (2006) Combined immunohistochemistry of beta-catenin, cytokeratin 7, and cytokeratin 20 is useful in discriminating primary lung adenocarcinomas from metastatic colorectal cancer. BMC Cancer 6:31

    Google Scholar 

  • Israeli RS, Powell CT, Corr JG, Fair WR, Heston WDW (1994) Expression of the prostate-specific membrane antigen. Cancer Res 54:1807–1811

    PubMed  CAS  Google Scholar 

  • Jorgensen JT, Nielsen KV, Ejlertsen B (2007) Pharmacodiagnostics and targeted therapies—a rational approach for individualizing medical anticancer therapy in breast cancer. Oncologist 12:397–405

    Article  PubMed  Google Scholar 

  • Leader M, Patel J, Makin C, Henry K (1986) An analysis of the sensitivity and specificity of the cytokeratin marker CAM 5.2 for epithelial tumours. Results of a study of 203 sarcomas, 50 carcinomas and 28 malignant melanomas. Histopathology 10:1315–1324

    Article  PubMed  CAS  Google Scholar 

  • Martignoni G et al (2004) CD10 is expressed in a subset of chromophobe renal cell carcinomas. Mod Pathol 17:1455–1463

    Article  PubMed  CAS  Google Scholar 

  • Miettinen M, Lasota J (2006) Gastrointestinal stromal tumors: review on morphology, molecular pathology, prognosis, and differential diagnosis. Arch Pathol Lab Med 130:1466–1478

    PubMed  CAS  Google Scholar 

  • Molino A. et al (1995) Estrogen receptors in 699 primary breast cancers: a comparison of immunohistochemical and biochemical methods. Breast Cancer Res Treat 34:221–228

    Article  PubMed  CAS  Google Scholar 

  • Ordóñez NG (2000) Thyroid Transcription Factor-1 is a Marker of Lung and Thyroid Carcinomas. Adv Anat Pathol 7:123–127

    Article  PubMed  Google Scholar 

  • Ramos-Vara JA (2005) Technical aspects of immunohistochemistry. Vet Pathol 42:405–426

    Article  PubMed  CAS  Google Scholar 

  • Ratushny V, Astsaturov I, Burtness BA, Golemis EA, Silverman JS (2009) Targeting EGFR resistance networks in head and neck cancer. Cell Signal 21:1255–1268

    Article  PubMed  CAS  Google Scholar 

  • Riddle ND, Quigley BC, Browarsky I, Bui MM (2010) Leiomyosarcoma arising in the pancreatic duct: a case report and review of the current literature. Case Report Med 2010:252–364

    Google Scholar 

  • Ridolfi RL, Jamehdor MR, Arber JM (2000) HER-2/neu testing in breast carcinoma: a combined immunohistochemical and fluorescence in situ hybridization approach. Mod Pathol 13:866–873

    Article  PubMed  CAS  Google Scholar 

  • Rossi G et al (2004) TTF-1, Cytokeratin 7, 34βE12, and CD56/NCAM Immunostaining in the subclassification of large cell carcinomas of the lung. Am J Clin Pathol 122:884–893

    Article  PubMed  CAS  Google Scholar 

  • Scartozzi M et al (2004) Epidermal Growth Factor Receptor (EGFR) Status in primary colorectal tumors does not correlate with egfr expression in related metastatic sites: implications for treatment with egfr-targeted monoclonal antibodies. J Clin Oncol 22:4772–4778

    Article  PubMed  CAS  Google Scholar 

  • Schlom J (1986) Basic Principles and applications of monoclonal antibodies in the management of carcinomas: the Richard and Hinda Rosenthal Foundation award lecture. Cancer Res 46:3225–3238

    PubMed  CAS  Google Scholar 

  • Siebert JD, McClure SP, Banks PM, Gulley ML (1995) Hodgkin’s disease, mixed cellularity type, with a B-cell immunophenotype. Report of a case and literature review. Arch Pathol Lab Med 119:474–479

    PubMed  CAS  Google Scholar 

  • Sigel JE, Hsi ED (2000) Immunohistochemical analysis of CD30-positive lymphoproliferative disorders for expression of CD95 and CD95L. Mod Pathol 13:446–451

    Article  PubMed  CAS  Google Scholar 

  • Sternberger LA, Sternberger NH (1986) The unlabeled antibody method: comparison of peroxidase-antiperoxidase with avidin-biotin complex by a new method of quantification. J Histochem Cytochem 34:599–605

    Article  PubMed  CAS  Google Scholar 

  • Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333

    Article  PubMed  CAS  Google Scholar 

  • van Doorn HC, Burger CW, van der Valk P, Bonfrér HMG (2000) Oestrogen, progesterone, and androgen receptors in ovarian neoplasia: correlation between immunohistochemical and biochemical receptor analyses. J Clin Pathol 53:201–205

    Article  PubMed  CAS  Google Scholar 

  • Webster JD, Miller MA, Dusold D, Ramos-Vara J (2009) Effects of prolonged formalin fixation on diagnostic immunohistochemistry in domestic animals. J Histochem Cytochem 57:753–761

    Article  PubMed  CAS  Google Scholar 

  • Yilmazer D, Han Ü, Önal B (2007) A comparison of the vascular density of VEGF expression with microvascular density determined with CD34 and CD31 staining and conventional prognostic markers in renal cell carcinoma. Int Urol Nephrol 39:691–698

    Article  PubMed  CAS  Google Scholar 

  • Zubovits J, Buzney E, Yu L, Duncan LM (2004) HMB-45, S-100, NK1/C3, and MART-1 in metastatic melanoma. Hum Pathol 35:217–223

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Centre Hospitalier Affilié Universitaire de Québec, Hôpital l’Enfant-Jésus, Faculty of Medicine, Laval University, Quebec, QC, Canada

    Dr. Mohib Wadie Morcos MD

Authors
  1. Dr. Mohib Wadie Morcos MD
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Correspondence to Mohib Wadie Morcos MD .

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Editors and Affiliations

  1. Centre for Genomic Regulation, N/A, Barcelona, N/A, Spain

    Julia V. Burnier

  2. , Ophthalmology, Pathology, Medicine & Onc, McGill University Health Centre, University Street 3775, Montreal, H3A 2B4, Québec, Canada

    Miguel N. Burnier, Jr.

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Morcos, M. (2013). Diagnostic Immunohistochemistry in Tumor Metastasis. In: Burnier, J., Burnier, Jr., M. (eds) Experimental and Clinical Metastasis. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3685-0_21

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