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Yop Effector Proteins from Yersinia pseudotuberculosis Impair Dendritic Cell Activation

  • Aline Tansini
  • Felipe Fortino Verdan da Silva
  • Rubens Rodrigues dos SantosJr.
  • Marisa Campos Polesi Placeres
  • Beatriz Maria Machado de Medeiros
  • Iracilda Zeppone Carlos
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 954)

Abstract

Yersinia pseudotuberculosis can inhibit multiple stages of dendritic cell (DC) function, including antigen uptake and degradation, maturation, and subsequent T-cell activation. In this study, we analyzed whether Y. pseudotuberculosis infection and Yop effector proteins play a role in the maturation and immunostimulatory capacity of DCs originating from differentiated bone marrow precursors from BALB/c or C57BL/6 mice. We found that Y. pseudotuberculosis reduces major histocompatibility complex (MHC) class II, CD80 and CD86 expression in both strains of mice, although the bacteria had a greater effect in BALB/c mice. We observed that YopE could interfere with the expression of maturation markers, which indicates a possible role for YopE in the inhibition of DC maturation. Infection by Y. pseudotuberculosis leads to a decrease in the immunostimulatory capacity of DCs, with T-cell proliferation significantly reduced on day 4 of co-culture. Stimulation of T cells by DCs infected with YopJ, YopE, and YopH mutants has a less robust effect on T-cell proliferation. The reduction in the expression of maturation markers and diminished immunostimulatory capacity of infected DCs may contribute to the ability of Y. pseudotuberculosis to evade adaptive immune responses and subsequently inhibit the host’s ability to clear the infection.

Keywords

Major Histocompatibility Complex Class Dendritic Cell Maturation Dendritic Cell Function Maturation Marker Surface Molecule Expression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo FAPESP/Brazil (grant number 09/00984-5) and Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq/Brazil.

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Aline Tansini
    • 1
  • Felipe Fortino Verdan da Silva
    • 1
  • Rubens Rodrigues dos SantosJr.
    • 1
  • Marisa Campos Polesi Placeres
    • 1
  • Beatriz Maria Machado de Medeiros
    • 1
  • Iracilda Zeppone Carlos
    • 1
  1. 1.UNESP—Universidade Estadual PaulistaFaculdade de Ciências FarmacêuticasAraraquaraBrazil

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