Abstract
Plague is a zoonotic disease caused by Yersinia pestis, the etiological agent of pneumonic and bubonic plague. Thirty-two individuals were vaccinated with Y. pestis EV line NIIEG and were evaluated over a 1-month period. The number of leukocytes and lymphocytes (T-helper cells, cytotoxic lymphocytes, and B lymphocytes), the total IgG, IgM, and IgE content, and the phagocytic activity of cells isolated from the vaccinated donors were examined. We also evaluated specific humoral anti-plague immunity using the F1 antigen. We analyzed changes in IFN-γ synthesis and the expression of the cell surface markers CD45RO, HLA-DR, and CD69 in both the total T-lymphocyte population and the T-helper cell subpopulation after in vitro activation with the F1 antigen. Our results demonstrate that the activation indices of the CD3+CD4+CD69+ and CD3+CD4+IFN-γ+ T-helper subpopulations can be used to assess anti-plague-specific cellular immunity, supplementing the assessment of humoral immunity.
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Firstova, V.V., Tyurin, E.A., Kravchenko, T.B., Zyrina, E.V., Biketov, S.F., Dyatlov, I.A. (2012). The In Vitro Evaluation of Anti-Plague Cellular Immunity by Quantitative Analysis of IFN-γ Synthesis and the Appearance of Activation Molecules on the Surface of T-Helper Cells. In: de Almeida, A., Leal, N. (eds) Advances in Yersinia Research. Advances in Experimental Medicine and Biology, vol 954. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3561-7_22
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DOI: https://doi.org/10.1007/978-1-4614-3561-7_22
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