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Isolation of Enteropathogenic Yersinia from Non-human Sources

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Advances in Yersinia Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 954))

Abstract

Isolation of enteropathogenic Yersinia from non-human sources is tricky, laborious and time consuming especially due to the small number of pathogenic Yersinia in asymptomatic carriers and the large number of psychrotrophic bacteria in food and environmental samples. Several different methods have been applied for isolation of Yersinia enterocolitica from non-human samples. Direct plating on selective agar plates is useful for detection of high levels (>104 CFU/g) of Yersinia in the sample. Enrichment in high-selective broth is needed for isolation of Y. enterocolitica from food and environmental samples. Cold enrichment at 4°C for 1–3 weeks is suitable for simultaneous isolation of Y. enterocolitica and Yersinia pseudotuberculosis especially when animal carriers are studied. So far, no efficient methods are available for isolation of enteropathogenic Yersinia in food and environmental samples. Thus, PCR is a useful method to use in parallel with culture methods to screen enteropathogenic Yersinia in food and environmental samples. Accurate identification of Yersinia strains is crucial for epidemiological studies. However, phenotypic tests are not sufficient for correct identification of enteropathogenic Yersinia spp. Thus, effort should be made to develop better methods for isolation and accurate identification of enteropathogenic Yersinia from non-human samples.

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Authors and Affiliations

  1. Department of Food Hygiene and Environmental Health, University of Helsinki, 66, FI-00014, Helsinki, Finland

    Maria Fredriksson-Ahomaa

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  1. Maria Fredriksson-Ahomaa
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Correspondence to Maria Fredriksson-Ahomaa .

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  1. , Centro de Pesquisa Aggeu Magalhães, Campus da UFPE, Recife, 50670, Brazil

    Alzira Maria Paiva de Almeida

  2. , Centro de Pesquisa Aggeu Magalhães, Campus da UFPE, Recife, 50670-420, Brazil

    Nilma Cintra Leal

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Fredriksson-Ahomaa, M. (2012). Isolation of Enteropathogenic Yersinia from Non-human Sources. In: de Almeida, A., Leal, N. (eds) Advances in Yersinia Research. Advances in Experimental Medicine and Biology, vol 954. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3561-7_12

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