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Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes

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Laboratory Protocols in Fungal Biology

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Fungi are important pathogens of human diseases, as well as to agricultural crop and trees. Molecular diagnostics can detect diseases early, and improve identification accuracy and follow-up disease management. The use of padlock probe is effective to facilitate these detections and pathogen identification quickly and accurately. In this chapter we describe three diagnostic assays that utilize padlock probes in combination with various technologies for the detection of pathogenic fungi.

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Acknowledgements

We are grateful to Drs. N. Saksena, S. Chen, and F. Kong (Westmead Hospital, Australia) for assistance and advice. Part of the funding for this research has been provided by Genome Canada and Genome British Columbia in support of The Tria I and Tria II Projects http://www.thetriaproject.ca.

Author information

Authors and Affiliations

  1. Department of Forest Sciences, The University of British Columbia, 2424 Main Mall, Vancouver, BC, Canada, V6T 1Z4

    Clement K. M. Tsui & Richard C. Hamelin

  2. Centre of Virus Research, Westmead Millennium Institute, University of Sydney, Westmead Hospital, Darcy Road, Westmead, NSW, 2145, Australia

    Bin Wang

  3. Department of Bio-Interactions and Plant Health, Plant Research International B. V, Droevendaalsesteeg 1, Wageningen, 6708PB, The Netherlands

    Cor D. Schoen

  4. Natural Resources Canada, Laurentian Forestry Centre, 1055 rue du P.E.P.S, Quebec, QC, Canada, G1V 4C7

    Richard C. Hamelin

Authors
  1. Clement K. M. Tsui
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  2. Bin Wang
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  3. Cor D. Schoen
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  4. Richard C. Hamelin
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Editor information

Editors and Affiliations

  1. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Vijai Kumar Gupta

  2. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Maria G. Tuohy

  3. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Manimaran Ayyachamy

  4. , Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, The Pfizer Biotech Campus at Grange Castle, Clondalkin, N/A, Dublin, Ireland

    Kevin M. Turner

  5. , School of Natural Sciences, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Anthonia O’Donovan

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Tsui, C.K.M., Wang, B., Schoen, C.D., Hamelin, R.C. (2013). Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_49

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