Abstract
A method based on solid phase microextraction coupled to gas chromatography–mass spectrometry (GC–MS) for the detection and identification of microbial volatile organic compounds (MVOCs) in the headspace of filamentous fungi is presented. MVOCs are identified by comparison of mass spectra and linear temperature programmed retention indices (LTPRIs) with database entries and LTPRIs published in literature. The presented method enables the monitoring of the formation of volatile metabolites for defined time intervals during cultivation of the investigated fungus. The experimental procedure is exemplified with Fusarium graminearum and Trichoderma atroviride but can also be used to detect, identify and profile MVOCs produced by other filamentous fungi.
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The financial support by the Austrian Science Fund (FWF projects F3702 and F3706) is gratefully acknowledged.
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Kluger, B., Zeilinger, S., Wiesenberger, G., Schöfbeck, D., Schuhmacher, R. (2013). Detection and Identification of Fungal Microbial Volatile Organic Compounds by HS-SPME-GC–MS. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_42
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DOI: https://doi.org/10.1007/978-1-4614-2356-0_42
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