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Laboratory Protocols in Fungal Biology pp 337–341Cite as

Quantitative Sampling Methods for the Analysis of Fungi: Air Sampling

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Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Quantitative sampling of fungi can be carried out in a variety of ways for a large array of purposes. These include approaches such as tape sampling, settled dust sampling, bulk material sampling, and air sampling followed by macroscopic analysis, polymerase chain reaction (PCR), or immunochemical methods for quantitation. Air sampling is widely used in a variety of industries and settings as a means of isolating material for the identification and potential enumeration of fungal strains thus we have chosen to discuss this approach to sampling in detail in this chapter. PCR overcomes the limitations of traditional culturing and macroscopic methods as it is not dependant on culturability or viability of the microorganisms. This chapter discusses a general approach to carrying out such air sampling and PCR-based quantitation.

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Author information

Authors and Affiliations

  1. Department of Life Sciences, University of Limerick, Castletroy, Limerick, Ireland

    Mary C. O’Loughlin

  2. Centre for Chromosome Biology, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

    Katherine D. Turner

  3. Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, The Pfizer Biotech Campus at Grange Castle, Clondalkin, Dublin, 22, Ireland

    Kevin M. Turner

Authors
  1. Mary C. O’Loughlin
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  2. Katherine D. Turner
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  3. Kevin M. Turner
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Corresponding author

Correspondence to Mary C. O’Loughlin .

Editor information

Editors and Affiliations

  1. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Vijai Kumar Gupta

  2. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Maria G. Tuohy

  3. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Manimaran Ayyachamy

  4. , Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, The Pfizer Biotech Campus at Grange Castle, Clondalkin, N/A, Dublin, Ireland

    Kevin M. Turner

  5. , School of Natural Sciences, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Anthonia O’Donovan

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O’Loughlin, M.C., Turner, K.D., Turner, K.M. (2013). Quantitative Sampling Methods for the Analysis of Fungi: Air Sampling. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_29

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