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Protocol for the In Vivo Quantification of Superoxide Radical in Fungi

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Laboratory Protocols in Fungal Biology

Part of the book series: Fungal Biology ((FUNGBIO))

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Abstract

The presented protocol for superoxide radical in vivo quantification in fungal tissues is based on the quantification of 2-hydroxyethidium (2-OH-E+) after its isolation and fluorometric quantitation. The protocol is ultrasensitive (i.e., <1 pmol) and applicable to any kind of fungal tissue; it can also be used for in vitro studies.

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References

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Authors and Affiliations

  1. Department of Biology, University of Patras, University Campus, 25600, Patras, Greece

    Konstantinos Grintzalis, Ioannis Papapostolou & Christos Georgiou

Authors
  1. Konstantinos Grintzalis
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  2. Ioannis Papapostolou
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  3. Christos Georgiou
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Corresponding author

Correspondence to Christos Georgiou .

Editor information

Editors and Affiliations

  1. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Vijai Kumar Gupta

  2. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Maria G. Tuohy

  3. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Manimaran Ayyachamy

  4. , Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, The Pfizer Biotech Campus at Grange Castle, Clondalkin, N/A, Dublin, Ireland

    Kevin M. Turner

  5. , School of Natural Sciences, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Anthonia O’Donovan

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Grintzalis, K., Papapostolou, I., Georgiou, C. (2013). Protocol for the In Vivo Quantification of Superoxide Radical in Fungi. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_20

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