Histopathological Technique for Detection of Fungal Infections in Plants

Chapter
Part of the Fungal Biology book series (FUNGBIO)

Abstract

Microscopic examination of the interaction between pathogenic fungi and their host plants has been instrumental in deciphering the biology of this relationship and can serve as a useful diagnostic tool. In this chapter, we describe the technique of fixing fungal infections of plant samplings for histopathological experiments. Toluidine blue O’ staining methods coupled with stereoscopic microscopy are used to scan the infection structures of the fungus Fusarium spp. and host response in Psidium guajava L. root tissues.

Keywords

Fungal infections Histopathological experiments Microscopy Staining techniques TOLUIDINE blue O’ Fusarium spp. Psidium guajava L. 

Notes

Acknowledgements

The authors are very grateful to Director, CISH; Head, Department of Crop-Protection, Central Institute of Subtropical Horticulture (CISH), Lucknow; and Prof. Shakti Baijal, Ex-Dean, FASC, MITS University, Rajasthan for providing the necessary research grants.

References

  1. 1.
    Johansen DA (1940) Plant microtechnique. McGraw-Hill, New YorkGoogle Scholar
  2. 2.
    Meyberg M (1988) Selective staining of fungal hyphae in parasitic and symbiotic plant-fungus associations. Histochemistry 88:197–199PubMedCrossRefGoogle Scholar
  3. 3.
    Pandey BK (1984) Studies of chickpea blight caused by Ascophyta rafiei (Pass) Labr. with special reference to survival in crop debris [PhD thesis]. Department of Plant Pathology. G. B. Pant University of Agriculture & Technology, Pantnagar, UP, IndiaGoogle Scholar
  4. 4.
    Saha DC, Jackson MA, Johnson-Cicalese JM (1988) A rapid staining method for detection of endophytic fungi in turf and forage grasses. Phytopathology 78:237–239CrossRefGoogle Scholar
  5. 5.
    Gupta VK, Misra AK, Pandey BK (2012) Histopathological changes during wilting in guava root. Arch Phytopathol Plant Protect 45(5):570–573 Available from http://www.tandfonline.com/doi/abs/10.1080/03235408.2011.588047Google Scholar
  6. 6.
    Knight NL, Sutherland MW (2001) A rapid differential staining technique for Fusarium pseudograminearum in cereal tissues during crown rot infections. Plant Pathol Online. First-doi: 10.1111/j.1365-3059.2011.02462.x
  7. 7.
    Jensen WA (1962) Botanical histochemistry: principals and practices. WH Freeman, San Francisco, LondonGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Molecular Glycobiotechnology Group, Department of Biochemistry, School of Natural SciencesNational University of Ireland GalwayGalwayIreland
  2. 2.Department of Science, Faculty of Arts, Science & CommerceMITS UniversitySikarIndia
  3. 3.Molecular Plant Pathology Laboratory, Central Institute for Subtropical HorticultureIndian Council of Agricultural ResearchLucknowIndia

Personalised recommendations