Histopathological Technique for Detection of Fungal Infections in Plants

  • Vijai Kumar Gupta
  • Brejesh Kumar Pandey
Part of the Fungal Biology book series (FUNGBIO)


Microscopic examination of the interaction between pathogenic fungi and their host plants has been instrumental in deciphering the biology of this relationship and can serve as a useful diagnostic tool. In this chapter, we describe the technique of fixing fungal infections of plant samplings for histopathological experiments. Toluidine blue O’ staining methods coupled with stereoscopic microscopy are used to scan the infection structures of the fungus Fusarium spp. and host response in Psidium guajava L. root tissues.


Fungal infections Histopathological experiments Microscopy Staining techniques TOLUIDINE blue O’ Fusarium spp. Psidium guajava L. 



The authors are very grateful to Director, CISH; Head, Department of Crop-Protection, Central Institute of Subtropical Horticulture (CISH), Lucknow; and Prof. Shakti Baijal, Ex-Dean, FASC, MITS University, Rajasthan for providing the necessary research grants.


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© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Molecular Glycobiotechnology Group, Department of Biochemistry, School of Natural SciencesNational University of Ireland GalwayGalwayIreland
  2. 2.Department of Science, Faculty of Arts, Science & CommerceMITS UniversitySikarIndia
  3. 3.Molecular Plant Pathology Laboratory, Central Institute for Subtropical HorticultureIndian Council of Agricultural ResearchLucknowIndia

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