Histopathological Technique for Detection of Fungal Infections in Plants
Microscopic examination of the interaction between pathogenic fungi and their host plants has been instrumental in deciphering the biology of this relationship and can serve as a useful diagnostic tool. In this chapter, we describe the technique of fixing fungal infections of plant samplings for histopathological experiments. Toluidine blue O’ staining methods coupled with stereoscopic microscopy are used to scan the infection structures of the fungus Fusarium spp. and host response in Psidium guajava L. root tissues.
KeywordsFungal infections Histopathological experiments Microscopy Staining techniques TOLUIDINE blue O’ Fusarium spp. Psidium guajava L.
The authors are very grateful to Director, CISH; Head, Department of Crop-Protection, Central Institute of Subtropical Horticulture (CISH), Lucknow; and Prof. Shakti Baijal, Ex-Dean, FASC, MITS University, Rajasthan for providing the necessary research grants.
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