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Use of Fourier-Transform Infrared (FTIR) Microscopy Method for Detection of Phyto-Fungal Pathogens

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Laboratory Protocols in Fungal Biology

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Reliable and rapid identification of the fungal pathogens that cause plant diseases is playing an important role in their control strategies. The available methods for identification of fungi are time-consuming and not always very specific. Fourier-transform infrared microscopy is proving to be a reliable and sensitive method for detection of molecular changes in cells. Fungi pathogens display typical infrared spectra that differ from spectra of substrate material such as potato, which make it is possible to detect and identify such pathogens directly from the infected tissue.

In addition, although different strains of the same fungi species display very similar infrared spectra, there are specific spectral differences between them that might be successfully used, with the assistance of advanced statistical methods, for the identification of these fungal strains.

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Author information

Authors and Affiliations

  1. Analytical Equipment Unit, Ben-Gurion University of the Negev, 653, Beer-Sheva, 84105, Israel

    Vitaly Erukhimovitch

  2. Department of Virology and Developmental Genetics, Ben-Gurion University of the Negev, 653, Beer-Sheva, 84105, Israel

    Mahmoud Huleihel

Authors
  1. Vitaly Erukhimovitch
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  2. Mahmoud Huleihel
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Corresponding author

Correspondence to Mahmoud Huleihel .

Editor information

Editors and Affiliations

  1. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Vijai Kumar Gupta

  2. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Maria G. Tuohy

  3. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Manimaran Ayyachamy

  4. , Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, The Pfizer Biotech Campus at Grange Castle, Clondalkin, N/A, Dublin, Ireland

    Kevin M. Turner

  5. , School of Natural Sciences, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Anthonia O’Donovan

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Erukhimovitch, V., Huleihel, M. (2013). Use of Fourier-Transform Infrared (FTIR) Microscopy Method for Detection of Phyto-Fungal Pathogens. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_10

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