Abstract
Acetate as precursor and tracer for cerebral metabolism has received high interest since the late 1950s when it was first shown with radiolabels that, among others, acetate incorporates very differently into cerebral metabolites than glucose. Subsequent work has taken advantage of acetate’s exclusive cerebral uptake into glial cells in order to probe metabolic compartmentation and the interplay of the intricately and inextricably intermingled glial and neuronal tissues in intact brain. The present review takes a three-pronged approach to outline the current understanding of cerebral acetate uptake, metabolism and the learning derived thereof with regard to the integral metabolism in mammalian brain. (1) Acetate-based tracer modalities including radiography, positron emission tomography (PET) and magnetic resonance spectroscopy (MRS) together with corresponding labelling concepts ranging from specific (radio-)activity to advanced multi-labelling strategies used for assessing cerebral metabolism are presented. (2) Translational aspects and efforts in moving the assessment of cerebral acetate metabolism from cell cultures and ex vivo tissue toward its non-invasive detection in situ in the brain of living animals and man are then discussed. (3) The original notion of cerebral substrate selection is complemented with current data on metabolic compartmentation and substrate trafficking to build a comprehensive, though in part still controversial view on cerebral metabolism. A collective of studies, which have utilised acetate to explore cerebral metabolism in health and disease, are put into perspective with this latter notion. Metabolism in brain tumours and cerebral ischemia as well as alterations in cerebral metabolism brought about by primarily extra-cerebral disorders such as diabetes and thyroid hormone deficits are discussed. Finally, acetate’s involvement in psychiatric and neurological disorders receives particular focus as this area is largely dominated by newer work addressing neurotransmitter balances and receptor involvement from a glial stance and is offering potential links to functional and behavioural data.
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Badar-Goffer RS, Bachelard HS, Morris PG (1990) Cerebral metabolism of acetate and glucose studied by 13C-n.m.r. spectroscopy: a technique for investigating metabolic compartmentation in the brain. Biochem J 266:133–139
Badar-Goffer RS, Ben-Yoseph O, Bachelard HS, Morris PG (1992) Neuronal-glial metabolism under depolarizing conditions: a 13C-n.m.r study. Biochem J 282:225–230
Baslow MH (2003) N-Acetylaspartate in the vertebrate brain: metabolism and function. Neurochem Res 28:941–953
Berl S, Clarke DD (1983) The metabolic compartmentation concept. In: Hertz L, Kyamne E, McGeer G, Schousboe A (eds) Glutamine, glutamate and GABA in the central nervous system. Alan R. Liss Inc, New York, pp 205–217
Berl S, Nicklas WJ, Clarke DD (1968) Compartmentation of glutamic acid and metabilism in brain slices. J Neurochem 15:131–140
Blüml S, Moreno-Torres A, Shic F, Nguy C-H, Ross BD (2002) Tricarboxylic acid cycle of glia in the in vivo human brain. NMR Biomed 15:1–5
Bradford HF (1989) Glutamate, GABA and epilepsy. Epilepsia 30:17–25
Brand A, Richter-Landsberg C, Leibfritz D (1997) Metabolism of acetate in rat brain neurons, astrocytes and coculture: metabolic interactions between neurons and glia cells, monitored by NMR spectroscopy. Cell Mol Biol 43:645–657
Brenner E, Kondziella D, Haberg A, Sonnewald U (2005) Impaired glutamine metabolism in NMDA receptor hypofunction induced by MK801. J Neurochem 94:1594–1603
Brenner E, Sonnewald U, Schweitzer A, Andrieux A, Nehling A (2007) Hypoglutamatergic activity in the STOP knockout mouse: a potential model for chronic untreated schizophrenia. J Neurosci Res 85:3487–3493
Carroll PT (1997) Evidence to suggest that extracellular acetate is accumulated by rat hippocampal cholinergic nerve terminals for acetylcholine formation and release. Brain Res 753:47–55
Cerdan S, Künnecke B, Seelig J (1990) Cerebral metabolism of [1,2-13C2] acetate as determined by in vivo and in vitro 13C NMR. J Biol Chem 265:12916–12926
Chapa F, Künnecke B, Calvo R, del Rey FE, de Escobar GM, Cerdán S (1995) Adult-onset hypothyroidism and the cerebral metabolism of (1,2-13C2) acetate as detected by 13C nuclear magnetic resonance. Endocrinology 136:296–305
Chapa F, Cruz F, Garcia-Martin ML, Garcia-Espinosa MA, Cerdán S (2000) Metabolism of (1–13C) glucose and (2–13C, 2–2H3) acetate in the neuronal and glial compartments of the adult rat brain as detected by 13C, 2H NMR spectroscopy. Neurochem Int 37:217–228
Chateil J-F, Biran M, Thiaudière E, Canioni P, Merle M (2001) Metabolism of [1-13C] glucose and [2-13C] acetate in the hypoxic rat brain. Neurochem Int 38:399–407
Chowdhury GMI, Patel AB, Mason GF, Rothman DL, Behar KL (2007) Glutamatergic and GABAergic neurotransmitter cycling and energy metabolism in rat cerebral cortex during postnatal development. J Cereb Blood Flow Metab 27:1895–1907
Cremer JR, Braun LD, Oldendorf WH (1976) Changes duringdevelopment in transport processes of the blood-brain barrier. Biochim Biophys Acta 448:633–637
Cruz F, Scott SR, Brroso I, Santisteban P, Cerdán S (1998) Ontogeny and cellular location of the pyruvate recycling system in rat brain. J Neurochem 70:2613–2619
Cruz NF, Lasater A, Zielke HR, Dienel GA (2005) Activation of astrocytes in brain of conscious rats during acoustic stimulation: acetate utilization in working brain. J Neurochem 92:934–947
D’Adamo AF Jr, Yatsu FM (1966) Acetate metabolism in the nervous system. N-acetyl-L-aspartic acid and the biosynthesis of brain lipids. J Neurochem 13:961–965
de Graaf RA, Patel AB, Rothman DL, Behar KL (2006) Acute regulation of steady-state GABA levels following GABA-transaminase inhibition in rat cerebral cortex. Neurochem Int 48:508–514
De Koning-Verest IF (1980) Glutamate metabolism in ageing rat brain. Mech Ageing Develop 13:83–92
Dhopeshwarkar GA, Subramanian C, Mead JF (1971) Rapid uptake of [1-14C] acetate by the adult brain 15 seconds after carotid injection. Biochim Biophys Acta 248:41–47
Dienel GA, Popp D, Drew PD, Ball K, Krisht A, Cruz NF (2001a) Preferential labelling of glial and meningial brain tumors with [2-14C] acetate. J Nucl Med 42:1243–1250
Dienel GA, Liu K, Cruz NF (2001b) Local uptake of 14C-labeled acetate and butyrate in rat brain in vivo during spreading cortical depression. J Neurosci Res 66:812–820
Dienel GA, Cruz NF, Ball K, Popp D, Gokden M, Baron S, Wright D, Wenger GR (2003) Behavioural training increases local astrocytic metabolic activity but does not alter outcome of mild transient ischemia. Brain Res 961:201–212
Dolezal V, Tucek S (1981) Utilization of citrate, acetylcarnitine, acetate, pyruvate and glucose for the synthesis of acetylcholine in rat brain slices. J Neurochem 36:1323–1330
Eloqayli H, Dahl CB, Götestam KG, Unsgard G, Hadidi H, Sonnewald U (2001) Pentylenetetrazole decreases metabolic glutamate turnover in rat brain. J Neurochem 85:1200–1207
Eloqayli H, Dahl CB, Götestam KG, Unsgard G, Sonnewald U (2004) Changes of glial-neuronal interaction and metabolism after a subconvolsive dose of pentylenetetrazole. Neurochem Int 45:739–745
Eyjolfsson EM, Brenner E, Kondziella D, Sonnewald U (2006) Repeated injection of MK801: an animal model of schizophrenia. Neurochem Int 48:540–546
Freeman JM, Kossoff EH, Hartman AL (2007) The ketogenic diet: one decade later. Pediatrics 119:535–543
Garcia-Espinosa MA, Garcia-Martin ML, Cerdán S (2003) Role of glial metabolism in diabetic encephalopathy as detected by high resolution 13C NMR. NMR Biomed 16:440–449
Gonda O, Quastel JH (1966) Transport and metabolism of acetate in rat brain cortex in vitro. Biochem J 100:83–94
Haberg A, Qu H, Bakken IJ, Sande LM, White LR, Haraldseth O, Unsgard G, Aasly J, Sonnewald U (1998a) In vitro and ex vivo 13C-NMR spectroscopy studies of pyruvate recycling in brain. Dev Neurosci 20:389–398
Haberg A, Qu H, Haraldseth O, Unsgard G, Sonnewald U (1998b) In vivo injection of [1-13C] glucose and [1,2-13C] acetate combined with ex vivo 13C nuclear magnetic resonance spectroscopy: A novel approach to the study of middle cerebral artery occlusion in the rat. J Cereb Blood Flow Metab 18:1223–1232
Haberg A, Qu H, Haraldseth O, Unsgard G, Sonnewald U (2000) In vivo effects on adenosine A1 receptor agonist and antagonist on neuronal and astrocytic intermediary metabolism studied with ex vivo 13C NMR spectroscopy. J Neurochem 74:327–333
Haberg A, Qu H, Saether O, Unsgard G, Haraldseth O, Sonnewald U (2001) Differences in neurotransmitter synthesis and intermediary metabolism between glutamatergic and GABAergic neurons during 4 hours of middle cerebral artery occlusion in the rat: The role of astrocytes in neuronal survival. J Cereb Blood Flow Metab 21:1451–1463
Haberg A, Qu H, Sonnewald U (2006) Glutamate and GABA metabolism in transient and permanent middle cerebral artery occlusion in rat: importance of astrocytes for neuronal sutvival. Neurochem Int 48:531–540
Haberg A, Qu H, Hjelstuen MH, Sonnewald U (2007) Effect of the pyrrolopyrimidine lipid peroxidation inhibitor U-101033E on neuronal and astrocytic metabolism and infarct volume in rats with transient middle cerebral artery occlusion. Neurochem Int 50:932–940
Hammer J, Qu H, Haberg A, Sonnewald U (2001) In vivo effects of adenosine A2 receptor agonist and antagonist on neuronal and astrocytic intermediary metabolism studied with ex vivo 13C MR spectroscopy. J Neurochem 79:885–892
Hassel B, Sonnewald U (1995) Glial formation of pyruvate and lactate from TCA cycle intermediates: implications for the inactivation of transmitter amino acids? J Neurochem 65:2227–2234
Hassel B, Sonnewald U, Unsgard G, Fonnum F (1994) NMR spectroscopy of cultured astrocytes: effects of glutamine and the gliotoxin fluorocitrate. J Neurochem 62:2187–2194
Hassel B, Bachelard H, Jones P, Fonnum F, Sonnewald U (1997) Trafficking of amino acids between neurons and glia in vivo. Effects of inhibition of glial metabolism by fluoroacetate. J Cereb Blood Flow Metab 17:1230–1238
Hirose S, Momosaki S, Hosoi R, Abe K, Gee A, Inoue O (2009) Role of NMDA receptor upon [14C] acetate uptake into intact brain. Ann Nucl Med 23:143–147
Hosoi R, Okada M, Hatazawa J, Gee A, Inoue O (2004) Effect of astrocytic energy metabolism depressant on 14C-acetate uptake in intact rat brain. J Cereb Blood Flow Metab 24:188–190
Hosoi R, Kashiwagi Y, Tokumura M, Abe K, Hatazawa J, Inoue O (2007) Sensitive reduction in 14C-acetate uptake in a short-term ischemic rat brain. J Stroke Cerebrovasc Dis 16:77–81
Hosoi R, Matsuyama Y, Hirose S-I, Koyama Y, Matsuda T, Gee A, Inoue O (2009) Characterization of 14C-acetate uptake in cultured rat astrocytes. Brain Res 1253:69–79
Inoue O, Hosoi R, Momosaki S, Yamamoto K, Amitani M, Yamaguchi M, Gee A (2006) Evaluation of [14C] phenylacetate as a prototype tracer for the measurement of glial metabolism in the rat brain. Nucl Med Biol 33:985–989
Johannessen CU, Petersen D, Fonnum F, Hassel B (2002) The acute effect of valproate on cerebral energy metabolism in mice. Epilepsy Res 47:147–256
Kondziella D, Qu H, Lüdemann W, Brinker T, Sletvold O, Sonnewald U (2003) Astrocyte metabolism is disturbed in the early development of experimental hydrocephalus. J Neurochem 85:274–281
Kondziella D, Brenner E, Eyjolfsson EM, Markinhuhta KR, Carlsson ML, Sonnewald U (2006) Glial-neuronal interactions are impaired in the schizophrenia model of repeated MK801 exposure. Neuropsychopharmacology 31:1880–1887
Kreis R, Hofmann L, Kuhlmann B, Boesch C, Bossi E, Hüppi PS (2002) Brain metabolite composition during early human brain development as measured by quantitative in vivo 1H magnetic resonance spectroscopy. Magn Reson Med 48:949–958
Künnecke B (1995) Application of 13C NMR spectroscopy to metabolic studies on animals. In: Beckmann N (ed) Carbon-13 NMR spectroscopy of biological systems. Academic, San Diego, pp 159–267
Künnecke B, Cerdan S (1989) Multilabeled 13C substrates as probes in in vivo 13C and 1H NMR spectroscopy. NMR Biomed 2:274–277
Künnecke B, Cerdan S, Seelig J (1993) Cerebral metabolism of [1,2-13C2] glucose and [U-13C4] 3-hydroxybutyrate in rat brain as detected by 13C NMR spectroscopy. NMR Biomed 6:264–277
Lajhta A, Berl S, Waelsch H (1959) Amino acids and protein metabolism of the brain. IV. The metabolism of glutamic acid. J Neurochem 3:322–332
Laptook AR, Peterson J, Porter AM (1988) Effects of lactic acid infusions and pH on cerebral blood flow and metabolism. J Cereb Blood Flow Metab 8:193–200
Lear JL, Ackermann RF (1990) Evaluation of radiolabeled acetate and fluoroacetate as potential tracers of cerebral oxidative metabolism. Metab Brain Dis 5:45–56
Lebon V, Petersen KF, Cline GW, Shen J, Mason GF, Dufour S, Behar KL, Shulman GI, Rothman DL (2002) Astroglial contribution to brain energy metabolism in humans revealed by 13C nuclear magnetic resonance spectroscopy: elucidation of the dominant pathway for neurotransmitter glutamate rpletion and measurement of astrocytic oxidative metabolism. J Neurosci 22:1523–1531
Li S, Chen Z, Zhang Y, Lizak M, Bacher J, Innis RB, Shen J (2005) In vivo single-shot, proton-localized 13C MRS of rhesus monkey brain. NMR Biomed 18:560–569
Liu R-S, Chang C-P, Chu L-S, Chu Y-K, Hsieh H-J, Chang C-W, Yang B-H, Yen S-H, Huamg M-C, Liao S-Q, Yeh S-H (2006) PET imaging of brain astrocytoma with 1-11C-acetate. Eur J Nucl Med Mol Imag 33:420–424
Martinez-Hernandez A, Bell KP, Norenberg MD (1977) Glutamine synthase: glial localization in brain. Science 195:1356–1357
Mason GF, Petersen KF, Lebon V, Rothman DL, Shulman GI (2006) Increased brain monocarboxylic acid transport and utilization in type 1 diabetes. Diabetes 55:929–934
Melo TM, Nehling A, Sonnewald U (2005) Metabolism is normal in astrocytes in chronically epileptic rats: a 13C NMR study of neuronal-glial interactions in a model of temporal lobe epilepsy. J Cereb Blood Flow Metab 25:1254–1264
Melo TM, Nehling A, Sonnewald U (2006a) Neuronal-glial interactions in rats fed a ketogenic diet. Neurochem Int 48:498–507
Melo TM, Sonnewald U, Touret M, Nehling A (2006b) Cortical glutamate metabolism is enhanced in a genetic model of absence epilepsy. J Cereb Blood Flow Metab 26:1496–1506
Melo TM, Sonnewald U, Bastholm IA, Nehling A (2007) Astrocytes may play a role in the etiology of absence epilepsy: a comparison between immature GAERS not yet expressing seizures and adults. Neurobiol Dis 28:227–235
Momosaki S, Hosoi R, Sanuki T, Todoroki K, Yamaguchi M, Gee A, Inoue O (2007) [14C] Benzyl acetate is a potential radiotracer for the measurement of glial metabolism in the rat brain. Nucl Med Biol 34:939–944
Muir D, Berl S, Clarke DD (1986) Acetate and fluoroacetate as possible markers for glial metabolism in vivo. Brain Res 380:336–340
Mukherji B, Sloviter HA (1973) Metabolism of acetate and N-acetylaspartate in isolated perfused rat brain. J Neurochem 20:633–636
Müller B, Qu H, Garseth M, White LR, Aasly J, Sonnewald U (2000) Amino acid neurotransmitter metabolism in neurons and glia following kainate injection in rats. Neurosci Lett 279:169–172
Nakamura R, Cheng S-C, Naruse H (1970) A study on the precursors of the acetyl moiety of acetylcholine in brain slices. Biochem J 118:443–450
Nishima M, Suzuki M, Matsushita K (2004) Trichinella spiralis: activity of the cerebral pyruvate recycling pathway of the host (mouse) in hypoglycemia induced by the infection. Exp Parasitol 106:62–65
Norenberg MD, Martinez-Hernandez A (1979) Fine structural localization of glutamine synthetase in astrocytes in brain. Brain Res 161:303–310
O’Neal RM, Koeppe RE, Williams EI (1966) Utilization in vivo of glucose and volatile fatty acids by sheep brain for the synthesis of acidic amino acids. Biochem J 101:591–597
Olstad E, Olsen GM, Sonnewald U (2007) Pyruvate recycling in cultured neurons from cerebellum. J Neurosci Res 85:3318–3325
Pascual JM, Carceller F, Roda JM, Cerdán S (1998) Glutamate, glutamine, and GABA as substrates for the neuronal and glial compartments after focal cerebral ischemia in rats. Stroke 29: 1048–1057
Patel AB, de Graaf RA, Mason GF, Rothman DL, Shulman RG, Behar KL (2005) The contribution of GABA to the glutamate/glutamine cycle and energy metabolism in the rat cortex in vivo. Proc Natl Acad Sci USA 102:5588–5593
Pierre K, Pellerin L (2005) Monocarboxylate transporters in the central nervous system: distribution, regulation and function. J Neurochem 94:1–14
Pierre K, Pellerin L, Debernardi R, Riederer BM, Magistretti PJ (2000) Cell-specific lacalization of monocarboxylate transporters, MCT1 and MCT2, in the adult mouse brain revealed by double immunohistochemical labeling and confocal microscopy. Neuroscience 100:617–627
Rae C, Hare N, Bubb WA, McEwan SR, Bröer A, McQuillan JA, Balcar VJ, Conigrave AD, Böer S (2003) Inhibition of glutamine transport depletes glutamate and GABA neurotransmitter pools: further evidence for metabolic compartmentation. J Neurochem 85:503–514
Rodrigues TB, Granado N, Ortiz O, Cerdán S, Moratella R (2007) Metabolic interaction between glutamatergic and dopaminergic neurotransmitter systems are mediated through D1 dopamine receptors. J Neurosci Res 85:3284–3293
Ross B, Lin A, Harris K, Bhattacharya P, Schweinsburg B (2003) Clinical experience with 13C MRS in vivo. NMR Biomed 16:358–369
Schousboe A (2003) Role of astrocytes in the maintenance and modulation of glutamatergic and GABAergic neurotransmission. Neurochem Res 28:347–352
Serres S, Bezancon E, Franconi J-M, Merle M (2007) Brain pyruvate recycling and peripheral metabolism: an NMR analysis ex vivo of acetate and glucose metabolism in the rat. J Neurochem 101:1428–1440
Serres S, Raffard G, Franconi J-M, Merle M (2008) Close coupling between astrocytic and neuronal metabolism to fulfill anaplerotic and energy needs in the rat brain. J Cereb Blood Flow Metab 28:712–724
Shank RP, Bennett GS, Freytag SO, Campbell GL (1985) Pyruvate carboxylase: astrocyte-specific enzyme implicated in the replenishment of amino acid neurotransmitter pools. Brain Res 329:364–367
Shen J, Petersen KF, Behar KL, Brown P, Nixon TW, Mason GF, Petroff OA, Shulman GI, Shulman RG, Rothman DL (1999) Determination of the rate of the glutamate/glutamine cycle in the human brain by in vivo 13C NMR. Proc Natl Acad Sci USA 97:8235–8240
Shic F, Ross BD (2003) Automated data processing of 1H-decoupled 13C MR spectra acquired from human brain in vivo. J Magn Reson 162:259–268
Sollenberg J, Sörbo B (1970) On the origin of the acetyl moiety of acetylcholine in brain studied with differential labelling technique using 3H-14C-mixed labelled glucose and acetate. J Neurochem 17:201–207
Sonnewald U, Westergaard N, Schousboe A, Svendsen JS, Unsgard G, Petersen SB (1993) Direct demonstration by [13C]NMR spectroscopy that glutamine from astrocytes is a precursor for GABA synthesis in neurons. Neurochem Int 22:19–29
Sonnewald U, Müller TB, Westergaard N, Unsgard G, Petersen SB, Schousboe A (1994) NMR spectroscopic study of cell cultures of astrocytes and neurons exposed to hypoxia: compartmentation of astrocyte metabolism. Neurochem Int 24:473–483
Sonnewald U, Akiho H, Koshiya K, Iwai A (1998) Effect of orotic acid on the metabolism of cortical astrocytes during hypoxia and reoxygenation: an NMR spectroscopy study. J Neurosci Res 51:103–108
Spence AM, Mankoff DA, Muzi M (2003) Positron emission tomography imaging of brain tumors. Neuroimag Clin N Am 13:717–739
Sze PY (1979) L-Glutamate decarboxylase. Adv Exp Med Biol 123:59–78
Tsuchida T, Takeuchi H, Okazawa H, Tsujikawa T, Fujibayashi Y (2008) Grading of brain glioma with 1-11C-acetate PET: comparison with 18F-FDG PET. Nucl Med Biol 35:171–176
Tucek S, Cheng S-C (1974) Provenance of the acetyl group of acetylcholine and compartmentation of acetyl-CoA and Krebs cycle intermediates in the brain in vivo. J Neurochem 22:893–914
Tyce GM, Ogg J, Owen CA Jr (1981) Metabolism of acetate to amino acids in brains of rats after complete hepatectomy. J Neurochem 36:640–650
Tyson RL, Gallagher C, Sutherland GR (2003) 13C-labelled substrates and the cerebral metabolic compartmentalization of acetate and lactate. Brain Res 992:43–52
Van den Berg CJ (1970) Compartmentation of glutamate metabolism in the developing brain: experiments with labelled glucose, acetate, phenylalanine, tyrosine and proline. J Neurochem 17:973–983
Van den Berg CJ, Garfinkel D (1971) A simulation study of brain compartments. Biochem J 123:211–218
Van den Berg CJ, Ronda G (1972) The incorporation of double-labelled acetate into glutamate and related amino acids from adult mouse brain: compartmentation of amino acid metabolism in brain. J Neurochem 27:1443–1448
Van den Berg CJ, Mela P, Waelsch H (1966) On the contribution of the tricarboxylic acid cycle to the synthesis of glutamate, glutamine and aspartate in brain. Biochem Biophys Res Commun 23:479–484
Van den Berg CJ, Krzalic LJ, Mela P, Waelsch H (1969) Compartmentation of glutamate metabolism in brain: evidence for the existence of two different tricarboxylic acid cycles in brain. Biochem J 113:281–290
Waniewski RA, Martin DL (1998) Preferential utilisation of acetate by astrocytes is attributable to transprt. J Neurosci 18:5225–5233
Wyss MT, Weber B, Treyer V, Heer S, Pellerin L, Magistretti PJ, Buck A (2009) Stimulation-induced increase of astrocytic oxidative metabolism in rats and humans investigated with 1-11C-acetate. J Cereb Blood Flow Metab 29:44–56
Yamamoto Y, Nishiyama Y, Kimura N, Kameyama R, Kawi N, Hatakeyama T, Kaji M, Ohkawa M (2008) 11C-Acetate PET in the evaluation of brain glioma: comparison with 11C-methionine and 18F-FDG-PET. Mol Imag Biol 10:281–287
Yang J, Li SS, Bacher J, Shen J (2007) Quantification of cortical GABA-glutamine cycling rate using in vivo magnetic resonance signal of [2-13C] GABA derived from glia-specific substrate [2-13C] acetate. Neurochem Int 50:371–378
Yoshimoto M, Waki A, Obata A, Furukawa T, Yonekura Y, Fujibayashi Y (2004) Radiolabeled choline as a proliferation marker: comparison with radiolabeled acetate. Nucl Med Biol 31:859–865
Yu ACH, Drejer J, Hertz L, Schousboe A (1983) Pyruvate carboxylase activity in primary cultures of astrocytes and neurons. J Neurochem 41:1484–1487
Yudkoff M, Daikhin Y, Nissim I, Horyn O, Lazarow A, Luhovyy B, Wehrli S, Nissim I (2005) Response of brain amino acid metabolism to ketosis. Neurochem Int 47:119–128
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Künnecke, B. (2012). Cerebral Acetate Metabolism: Towards Its In Vivo Assessment. In: Choi, IY., Gruetter, R. (eds) Neural Metabolism In Vivo. Advances in Neurobiology, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-1788-0_26
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