Skip to main content
SpringerLink
Log in
Book cover

Oxygen Transport to Tissue XXXIII pp 57–61Cite as

Influence of Stress Preconditioning on Hippocampal Neuronal Cell Death and Neurogenesis in Rat Cerebral Ischemia

  • Conference paper
  • First Online:

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 737))

Abstract

Stress-associated exacerbation of neuronal cell death following cerebral ischemia has been reported. Here, we evaluated the effect of stress prior to cerebral ischemia on neuronal cell death in rats. Male Sprague–Dawley rats were subjected to global ischemia. Four experimental groups were used (1) control, (2) ischemia, (3) stress, (4) ischemia following stress. A forced swimming test was used to impose stress. At 7 days after ischemia, we evaluated hippocampal neuronal cell death with Fluoro-Jade B staining and neurogenesis with BrdU staining. Hippocampal neuronal cell death in the ischemia group was significantly increased compared with that in the control group. The stress group also showed a significant increase of cell death compared with the control. The ischemia following stress group showed a much greater increase of hippocampal neuronal cell death than the other groups. As for neurogenesis, BrdU-positive cells in the ischemia group were significantly increased compared with the control and stress groups. In the ischemia following stress group, BrdU-positive cells were significantly decreased compared with the ischemia group. Thus, imposition of stress prior to cerebral ischemia appears to exacerbate neuronal cell death and to inhibit neurogenesis in rat hippocampus.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  1. Kinno T (1982) Delayed neuronal death in the gerbil hippocampus following ischemia. Brain Res 239:57–69

    Article  Google Scholar 

  2. Petito CK, Pulsinelli WA (1984) Delayed neuronal recovery and neuronal death in rat hippocampus following severe cerebral ischemia: possible relationship to abnormalities in neuronal processes. J Cereb Blood Flow Metab 4(2):194–205

    Article  PubMed  CAS  Google Scholar 

  3. Watanabe Y, Gould E, McEwen BS et al (1992) Stress induces atrophy of apical dendrites of hippocampal CA3 pyramidal neurons. Brain Res 588:341–345

    Article  PubMed  CAS  Google Scholar 

  4. Goldman SA, Nottebohm F (1983) Neuronal production, migration, and differentiation in a vocal control nucleus of the adult female canary brain. Proc Natl Acad Sci USA 80(8):2390–2394

    Article  PubMed  CAS  Google Scholar 

  5. Brown ES, Rush AJ, McEwen BS (1999) Hippocampal remodeling and damage by corticosteroids: implications for mood disorders. Neuropsychopharmacology 21(4):474–484

    Article  PubMed  CAS  Google Scholar 

  6. Parent JM (2003) Injury-induced neurogenesis in the adult mammalian brain. Neuroscientist 9:261–272

    Article  PubMed  Google Scholar 

  7. Smith ML, Auer RN, Siesjo BK (1984) The density and distribution of ischemic brain injury in the rat following 2–10 min forebrain ischemia. Acta Neuropathol (Berl) 64:319–332

    Article  CAS  Google Scholar 

  8. Cryan JF, Valentino RJ, Lucki I (2005) Assessing substrates underlying the behavioral effects of antidepressants using the modified rat forced swimming test. Neurosci Biobehav Rev 29(4–5):547–569

    Article  PubMed  CAS  Google Scholar 

  9. Sapolsky R (1996) Stress, glucocorticoids, and damage to the nervous system: the current state of confusion. Stress 1(1):1–19

    Article  PubMed  CAS  Google Scholar 

  10. Sapolsky R (1985) A mechanism for glucocorticoid toxicity in the hippocampus: increased neuronal vulnerabilityto metabolic insults. J Neurosci 5:1227

    Google Scholar 

  11. Wang SH, Zhang ZJ, Guo YJ et al (2008) Hippocampal neurogenesis and behavioural studies on adult ischemic rat response to chronic mild stress. Behav Brain Res 189:9–16

    Article  PubMed  Google Scholar 

  12. Smith MA, Makino S, Kvetnansky R et al (1995) Stress and glucocorticoids affect the expression of brain-derived neurotrophic factor and neurotrophin-3 mRNA in the hip- pocampus. J Neurosci 15:1768–1777

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Neurosurgery, Department of Neurological Surgery, Nihon University School of Medicine, 30-1 Oyaguchi-Kamimachi, Itabashi-ku, Tokyo, 173-8610, Japan

    Takashi Eriguchi, Nobuo Kutsuna, Yuko Kondo, Jie Shi, Yoshihiro Murata, Hideki Oshima, Kaoru Sakatani & Yoichi Katayama

  2. Division of Optical Brain Engineering, Department of Neurological Surgery, Nihon University School of Medicine, Tokyo, Japan

    Kaoru Sakatani

Authors
  1. Takashi Eriguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nobuo Kutsuna
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yuko Kondo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jie Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yoshihiro Murata
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hideki Oshima
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Kaoru Sakatani
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yoichi Katayama
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Takashi Eriguchi .

Editor information

Editors and Affiliations

  1. , Division of Neonatology, University Hospital Zurich, Frauenklinikstr. 10, Zurich, 8091, Switzerland

    Martin Wolf

  2. , Division of Neonatology, University Hospital Zurich, Frauenklinikstr. 10, Zurich, 8091, Switzerland

    Hans Ulrich Bucher

  3. Institute for Biomedical Engineering, University of Zurich/ETHZ, Wolfgang-Pauli-Strasse 27, Zurich, 8093, Switzerland

    Markus Rudin

  4. ESAT-SCD(SISTA), Dept. Elektrotechniek, Katholieke Universiteit Leuven, Kasteelpark Arenberg 10, Leuven-Heverlee, 3001, Belgium

    Sabine Van Huffel

  5. , Institute of Complementary Medicine KIKO, University of Bern, Imhoof-Pavillon, Inselspital, Bern, 3010, Switzerland

    Ursula Wolf

  6. Synthesizer Inc., Synthesizer Inc. 2773, Ellicott City, 21043, Maryland, USA

    Duane F. Bruley

  7. , Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, NE1 7RU, United Kingdom

    David K Harrison

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this paper

Cite this paper

Eriguchi, T. et al. (2012). Influence of Stress Preconditioning on Hippocampal Neuronal Cell Death and Neurogenesis in Rat Cerebral Ischemia. In: Wolf, M., et al. Oxygen Transport to Tissue XXXIII. Advances in Experimental Medicine and Biology, vol 737. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-1566-4_9

Download citation

Publish with us

Policies and ethics

Search

Navigation