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Viral Molecular Machines pp 365–377Cite as

Lipid-Containing Viruses: Bacteriophage PRD1 Assembly

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 726))

Abstract

PRD1 is a tailless icosahedrally symmetric virus containing an internal lipid membrane beneath the protein capsid. Its linear dsDNA genome and covalently attached terminal proteins are delivered into the cell where replication occurs via a protein-primed mechanism. Extensive studies have been carried out to decipher the roles of the 37 viral proteins in PRD1 assembly, their association in virus particles and lately, especially the functioning of the unique packaging machinery that translocates the genome into the procapsid. These issues will be addressed in this chapter especially in the context of the structure of PRD1. We will also discuss the major challenges still to be addressed in PRD1 assembly.

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Acknowledgements

We would like to thank all our colleagues in the greasy phage field for their useful discussions over the years. This work was supported by the Academy of Finland Centre of Excellence Programme in Virus Research (2006–2011; 1129684 to SJB). V.M. is a fellow of the VGSB.

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Authors and Affiliations

  1. Structural Biology and Biophysics Programme, Institute of Biotechnology, University of Helsinki, 65, 00014, Helsinki, Finland

    Sarah J. Butcher & Violeta Manole

  2. Next Biomed Technologies NBT Oy, Biomedicum 2U, Tukholmankatu 8, 00290, Helsinki, Finland

    Nelli J. Karhu

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  1. Sarah J. Butcher
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  2. Violeta Manole
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  3. Nelli J. Karhu
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Correspondence to Sarah J. Butcher .

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Editors and Affiliations

  1. Dept. Biological Sciences, Purdue University, W. State St. 915, West Lafayette, 47907-2054, Indiana, USA

    Michael G. Rossmann

  2. Dept. Biology, Catholic University of America, Washington, 20064, District of Columbia, USA

    Venigalla B. Rao

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Butcher, S.J., Manole, V., Karhu, N.J. (2012). Lipid-Containing Viruses: Bacteriophage PRD1 Assembly. In: Rossmann, M., Rao, V. (eds) Viral Molecular Machines. Advances in Experimental Medicine and Biology, vol 726. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-0980-9_16

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