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The Potential Affibodies in New Cancer Marker Immunosensors

Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 109)

Abstract

In this paper, a simple and sensitive immunoassay for a tumor marker human epidermal growth factor receptor 2 (HER2) detection is presented. The immunoassay is based on a sandwich format in which modified magnetic microparticles were coupled to multiplexed electrochemical detection platform. The antibody modified beads are used to capture the protein from the sample solution and the sandwich assay is performed by adding Anti-ErbB2 affibody labeled with biotin. The enzyme alkaline phosphatase (AP) conjugated with streptavidin and its substrate (α-naphthyl-phosphate) were then used for the electrochemical detection by a multiplexed electrochemical platform. The immunosensor developed promise to be a sensitive, multiplexed tool for fast and easy HER2 multianalysis.

Keywords

Differential Pulse Voltammetry Sandwich Assay Affibody Molecule Enzyme Alkaline Phosphatase Human Epidermal Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Hoda Ilkhani
    • 1
    • 2
  • Marco Mascini
    • 3
  • Giovanna Marrazza
    • 3
  1. 1.Department of ChemistryUniversity of GuilanRashtIran
  2. 2.Center of Excellence in ElectrochemistryUniversity of TehranTehranIran
  3. 3.Dipartimento di Chimica “Ugo Schiff”Università degli Studi di FirenzeSesto FiorentinoItaly

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