Base-Calling for Bioinformaticians
High-throughput platforms execute billions of simultaneous sequencing reactions. Base-calling is the process of decoding the output signals of these reactions into sequence reads. In this chapter, we detail the facets of base-calling using the perspective of signal communication. We primarily focus on the Illumina high-throughput sequencing platform and review different third-party base-calling implementations.
KeywordsSupport Vector Machine Illumina Sequencing Residual Signal Illumina Platform Distortion Factor
The authors would like to thank Fabian Menges, Giuseppe Narzisi, and Bud Mishra for sharing their early TotalReCaller results, for Dan Valente for formulating the unified distortion model, and for Dina Esposito for useful comments on the chapter. Yaniv Erlich is an Andria and Paul Heafy family fellow.
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