Abstract
The high-throughput DNA sequencing technologies are based on immobilization of the DNA samples onto a solid support, cyclic sequencing reactions using automated fluidics devices, and detection of molecular events by imaging. Featured sequencing technologies include: GS FLX by 454 Life Technologies/Roche, Genome Analyzer by Solexa/Illumina, SOLiD by Applied Biosystems, CGA Platform by Complete Genomics, and PacBio RS by Pacific Biosciences. In addition, emerging technologies are discussed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bentley, D. R. 2006. Whole-genome re-sequencing. Curr Opin Genet Dev 16 (6):545–552. doi:S0959-437X(06)00208-5 [pii] 10.1016/j.gde.2006.10.009.
Bentley, DR, S Balasubramanian, HP Swerdlow, GP Smith, J Milton, CG Brown, KP Hall et al. 2008. Accurate whole human genome sequencing using reversible terminator chemistry. Nature 456:53–59.
Drmanac, R., A. B. Sparks, M. J. Callow, A. L. Halpern, N. L. Burns, B. G. Kermani, P. Carnevali et al. 2010. Human genome sequencing using unchained base reads on self-assembling DNA nanoarrays. Science 327 (5961):78–81. doi:1181498 [pii] 10.1126/science.1181498.
Eid, J, A Fehr, J Gray, K Luong, J Lyle, G Otto, P Peluso et al. 2009. Real-time DNA sequencing from single polymerase molecules. Science 323:133–138.
Flusberg, B. A., D. R. Webster, J. H. Lee, K. J. Travers, E. C. Olivares, T. A. Clark, J. Korlach, and S. W. Turner. 2010. Direct detection of DNA methylation during single-molecule, real-time sequencing. Nat Methods 7 (6):461–465. doi:nmeth.1459 [pii] 10.1038/nmeth.1459.
Korlach, J, A Bibillo, J Wegener, P Peluso, TT Pham, I Park, S Clark, GA Otto, and SW Turner. 2008. Long, processive enzymatic DNA synthesis using 100% dye-labeled terminal phosphate-linked nucleotides. Nucleosides Nucleotides Nucleic Acids 27:1072–1083.
Korlach, J., P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner. 2008. Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures. Proc Natl Acad Sci USA 105 (4):1176–1181.
Levene, M. J., J. Korlach, S. W. Turner, M. Foquet, H. G. Craighead, and W. W. Webb. 2003. Zero-mode waveguides for single-molecule analysis at high concentrations. Science 299 (5607):682–686.
Margulies, M., M. Egholm, W. E. Altman, S. Attiya, J. S. Bader, L. A. Bemben, J. Berka et al. 2005. Genome sequencing in microfabricated high-density picolitre reactors. Nature 437 (7057):376–380. doi:nature03959 [pii] 10.1038/nature03959.
Shendure, J., G. J. Porreca, N. B. Reppas, X. Lin, J. P. McCutcheon, A. M. Rosenbaum, M. D. Wang, K. Zhang, R. D. Mitra, and G. M. Church. 2005. Accurate multiplex polony sequencing of an evolved bacterial genome. Science 309 (5741):1728–1732. doi:1117389 [pii] 10.1126/science.1117389.
Smith, A. M., L. E. Heisler, R. P. St Onge, E. Farias-Hesson, I. M. Wallace, J. Bodeau, A. N. Harris et al. 2010. Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples. Nucleic Acids Res 38 (13):e142.
Travers, K. J., C. S. Chin, D. R. Rank, J. S. Eid, and S. W. Turner. 2010. A flexible and efficient template format for circular consensus sequencing and SNP detection. Nucleic Acids Res 38 (15):e159. doi:gkq543 [pii] 10.1093/nar/gkq543.
Valouev, A., J. Ichikawa, T. Tonthat, J. Stuart, S. Ranade, H. Peckham, K. Zeng et al. 2008. A high-resolution, nucleosome position map of C. elegans reveals a lack of universal sequence-dictated positioning. Genome Res 18 (7):1051–1063. doi:gr.076463.108 [pii] 10.1101/gr.076463.108.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this chapter
Cite this chapter
Myllykangas, S., Buenrostro, J., Ji, H.P. (2012). Overview of Sequencing Technology Platforms. In: Rodríguez-Ezpeleta, N., Hackenberg, M., Aransay, A. (eds) Bioinformatics for High Throughput Sequencing. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-0782-9_2
Download citation
DOI: https://doi.org/10.1007/978-1-4614-0782-9_2
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4614-0781-2
Online ISBN: 978-1-4614-0782-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)