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Biochemical Characterization of Cone Cyclic Nucleotide-Gated (CNG) Channel Using the Infrared Fluorescence Detection System

  • Xi-Qin DingEmail author
  • Alexander Matveev
  • Anil Singh
  • Naoka Komori
  • Hiroyuki Matsumoto
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 723)

Abstract

Cone vision mediated by photoreceptor cyclic nucleotide-gated (CNG) channel is essential for central and color vision and visual acuity. Cone CNG channel is composed of two structurally related subunit types, CNGA3 and CNGB3. Naturally occurring mutations in cone CNG channel are associated with a variety of cone diseases including achromatopsia, progressive cone dystrophy, and some maculopathies. Nevertheless, our understanding of the structure of cone CNG channel is quite limited. This is, in part, due to the challenge of studying cones in a rod-dominant mammalian retina. We have demonstrated a robust expression of cone CNG channel and a lack of rod CNG channel in the cone-dominant Nrl−/− retina and shown that the Nrl−/− mouse line is a valuable model to study cone CNG channel. This work examined the complex structure of cone CNG channel using infrared fluorescence Western detection combined with chemical cross-linking and blue native-PAGE. Our results suggest that the native cone CNG channel is a heterotetrameric complex likely at a stoichiometry of three CNGA3 and one CNGB3.

Keywords

Retina Photoreceptor Cone CNG channel Achromatopsia 

Notes

Acknowledgments

This work was supported by grants from the National Center for Research Resources (P20RR017703), the National Eye Institute (P30EY12190, R01EY019490, and R21EY17888), the American Health Assistance Foundation, and the Oklahoma Center for the Advancement of Science & Technology (OCAST). We thank Micaela J. Langevin for technical assistance.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Xi-Qin Ding
    • 1
    Email author
  • Alexander Matveev
    • 1
  • Anil Singh
    • 2
  • Naoka Komori
    • 2
  • Hiroyuki Matsumoto
    • 2
  1. 1.Department of Cell BiologyUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  2. 2.Department of Biochemistry and Molecular BiologyUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA

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