HSP70 Gene Expression in the Zebrafish Retina After Optic Nerve Injury: A Comparative Study Under Heat Shock Stresses

  • Chieko Fujikawa
  • Mikiko Nagashima
  • Kazuhiro Mawatari
  • Satoru KatoEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 723)


Unlike mammals, fish retinal ganglion cells (RGCs) can regrow their axon and restore visual function after optic nerve injury (ONI), which is the best model for CNS repair. Heat shock protein 70 (HSP70) plays an important role for cell protection by various environmental stresses. HSP70 is transactivated by phosphorylated Heat shock factor-1 (pHSF-1). In this study, we investigate the expression of HSP70 and pHSF-1 after ONI and heat shock condition (at 37°C for 30 min) in zebrafish (ZF) retina. After ONI, HSP70 was increased for a long time in the RGCs during 0.5–24 h and then returned to the control level by 72 h. In contrast, HSP70 increased for a short time in all nuclear layers at 0.5–1 h, and then returned to control level by 3 h after heat shock. The level of HSF-1 mRNA was significantly increased in ZF retina 0.5–24 h after ONI, but not after heat shock. pHSF-1 was detected after both heat shock and ONI. These results indicate that the increased expression of HSP70 mRNA after ONI is caused by both activations of translocation of pHSF-1 and of transcription of HSF-1 mRNA in the nucleus, whereas the increased expression of HSP70 mRNA after heat shock is caused only by activation of pHSF-1 translocation into the nucleus.


Heat Shock HSP70 mRNA cRNA Probe Heat Shock Element Optic Nerve Injury 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank to Ms Sachiko Higashi and Ms Tomoko Kano for secretarial and technical assistance.


  1. Airaksinen S, Jokilehto T, Råbergh CM et al (2003) Heat- and cold-inducible regulation of HSP70 expression in zebrafish ZF4 cells. Comp Biochem Physiol B Biochem Mol Biol 136:275–82PubMedCrossRefGoogle Scholar
  2. Attardi DG, Sperry RW (1963) Preferential selection of central pathways by regenerating optic fibers. Exp Neurol 7:46–64PubMedCrossRefGoogle Scholar
  3. Beckmann RP, Mizzen LE, Welch WJ (1990) Interaction of Hsp 70 with newly synthesized proteins: implications for protein folding and assembly. Science 248:850–854PubMedCrossRefGoogle Scholar
  4. Chiang HL, Terlecky SR, Plant CP et al (1989) A role for a 70-kilodalton heat shock protein in lysosomal degradation of intracellular proteins. Science 246:382–385PubMedCrossRefGoogle Scholar
  5. Kato S, Koriyama Y, Matukawa T et al (2007) Optic nerve regeneration in goldfish. In: Becker CG, Becker T (Eds) Model Organisms in Spinal Cord Regeneration. Weinheim 355–372Google Scholar
  6. Matsukawa T, Sugitani K, Mawatari K et al (2004) Role of purpurin as a retinol-binding protein in goldfish retina during the early stage of optic nerve regeneration: its priming action on neurite outgrowth. J Neurosci 24:8346–8353PubMedCrossRefGoogle Scholar
  7. Santoro MG (2000) Heat shock factors and the control of the stress response. Biochem Pharmacol 59:55–63PubMedCrossRefGoogle Scholar
  8. Shi Y, Thomas JO (1992) The transport of proteins into the nucleus requires the 70-kilodalton heat shock protein or its cytosolic cognate. Mol Cell Biol 12:2186–2192PubMedGoogle Scholar
  9. Sperry RW (1948) Patterning of central synapses in regeneration of the optic nerve in teleosts. Physiol Zool 21:351–361PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Chieko Fujikawa
    • 1
  • Mikiko Nagashima
    • 2
  • Kazuhiro Mawatari
    • 1
  • Satoru Kato
    • 3
    Email author
  1. 1.Division of Health Sciences, Graduate School of MedicineKanazawa UniversityKanazawaJapan
  2. 2.Department of Molecular Neurobiology, Division of Health Sciences, Graduate School of MedicineKanazawa UniversityKanazawaJapan
  3. 3.Department of Molecular Neurobiology, Graduate School of MedicineKanazawa UniversityKanazawaJapan

Personalised recommendations