Abstract
Confocal scanning laser ophthalmoscopy (SLO) and spectral-domain optical coherence tomography (OCT) are imaging modalities that have become increasingly popular among vision research groups worldwide. These devices are becoming easier to use and permit repeated in vivo imaging of intraocular tissues in small animal. We recently initiated baseline fundus imaging of all mice before their use in experimental studies. Here, we report that there are a substantial proportion of supposedly normal mice that have abnormal retinal morphology when evaluated with SLO and OCT. Our findings strongly indicate that mice should be prescreened with these imaging modalities before being used for experimental studies.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Eter N, Engel DR, Meyer L et al (2008) In vivo visualization of dendritic cells, macrophages, and microglial cells responding to laser-induced damage in the fundus of the eye. Invest Ophthalmol Vis Sci 49:3649–3658
Fischer MD, Huber G, Beck SC et al (2009) Noninvasive, in vivo assessment of mouse retinal structure using optical coherence tomography. PLoS One 4:e7507
Gabriele ML, Ishikawa H, Schuman JS et al (2010) Reproducibility of Spectral-Domain Optical Coherence Tomography Total Retinal Thickness Measurements in Mice. Invest Ophthalmol Vis Sci
Hawes NL, Smith RS, Chang B et al (1999) Mouse fundus photography and angiography: a catalogue of normal and mutant phenotypes. Mol Vis 5:22
Hollyfield JG, Bonilha VL, Rayborn ME et al (2008) Oxidative damage-induced inflammation initiates age-related macular degeneration. Nat Med 14:194–198
Huber G, Beck SC, Grimm C et al (2009) Spectral domain optical coherence tomography in mouse models of retinal degeneration. Invest Ophthalmol Vis Sci 50:5888–5895
Kocaoglu OP, Uhlhorn SR, Hernandez E et al (2007) Simultaneous fundus imaging and optical coherence tomography of the mouse retina. Invest Ophthalmol Vis Sci 48:1283–1289
Luhmann UF, Robbie S, Munro PM et al (2009) The drusenlike phenotype in aging Ccl2-knockout mice is caused by an accelerated accumulation of swollen autofluorescent subretinal macrophages. Invest Ophthalmol Vis Sci 50:5934–5943
Paques M, Guyomard JL, Simonutti M et al (2007) Panretinal, high-resolution color photography of the mouse fundus. Invest Ophthalmol Vis Sci 48:2769–2774
Ruggeri M, Tsechpenakis G, Jiao S et al (2009) Retinal tumor imaging and volume quantification in mouse model using spectral-domain optical coherence tomography. Opt Express 17:4074–4083
Xu H, Chen M, Manivannan A et al (2008) Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice. Aging Cell 7:58–68
Acknowledgments
The authors would like to thank the Foundation Fighting Blindness, Research to Prevent Blindness, Wolf Foundation, and NIH R01EY014240 for providing financial support. The authors also thank Dr. Nancy B. Bell for constructive input and assistance in the preparation of this chapter.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this paper
Cite this paper
Bell, B.A., Kaul, C., Rayborn, M.E., Hollyfield, J.G. (2012). Baseline Imaging Reveals Preexisting Retinal Abnormalities in Mice. In: LaVail, M., Ash, J., Anderson, R., Hollyfield, J., Grimm, C. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 723. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-0631-0_58
Download citation
DOI: https://doi.org/10.1007/978-1-4614-0631-0_58
Published:
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4614-0630-3
Online ISBN: 978-1-4614-0631-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)