Baseline Imaging Reveals Preexisting Retinal Abnormalities in Mice
Confocal scanning laser ophthalmoscopy (SLO) and spectral-domain optical coherence tomography (OCT) are imaging modalities that have become increasingly popular among vision research groups worldwide. These devices are becoming easier to use and permit repeated in vivo imaging of intraocular tissues in small animal. We recently initiated baseline fundus imaging of all mice before their use in experimental studies. Here, we report that there are a substantial proportion of supposedly normal mice that have abnormal retinal morphology when evaluated with SLO and OCT. Our findings strongly indicate that mice should be prescreened with these imaging modalities before being used for experimental studies.
KeywordsFundus Baseline Imaging Mice Retina Lesion Abnormalities Scanning laser Ophthalmoscope Optical coherence tomography
The authors would like to thank the Foundation Fighting Blindness, Research to Prevent Blindness, Wolf Foundation, and NIH R01EY014240 for providing financial support. The authors also thank Dr. Nancy B. Bell for constructive input and assistance in the preparation of this chapter.
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