Silencing the Expression of CTRP5/C1QTNF5 and ELOVL4 Genes by Small Interfering RNA

  • Venkata Ramana Murthy Chavali
  • Vidyullatha Vasireddy
  • Radha AyyagariEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 723)


Two monogenic macular degenerations, the stargardt-like macular degeneration (STGD3) and the late-onset retinal dystrophy (L-ORD), are caused due to mutations in the genes elongation of very long chain fatty acid-4 (ELOVL4) and C1Q tumor necrosis factor-related protein-5 (CTRP5/C1QTNF5) genes, respectively. It has been shown that disease-causing mutations alter the trafficking of these proteins and exert dominant-negative effect. Selective elimination of these mutant proteins may facilitate restoring the normal function of retinal tissue. In this study, we describe characterization of small interfering RNA (siRNA) probes that selectively and effectively knock down the expression of the wild-type and mutant ELOVL4 and the wild-type CTRP5 genes in Cos-7 cells. These probes will be valuable in studying the function of wild-type ELOVL4 and CTRP5 genes, affect of mutant proteins and the potential use of gene silencing in treating STGD3 and L-ORD.


CTRP5 C1QTNF5 ELOVL4 siRNA Small interfering RNA Late-onset retinal degeneration STGD3 L-ORD Stargardt-like macular degeneration 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Venkata Ramana Murthy Chavali
    • 1
  • Vidyullatha Vasireddy
    • 1
  • Radha Ayyagari
    • 1
    Email author
  1. 1.Department of Ophthalmology, Shiley Eye CenterUniversity of California San DiegoLa JollaUSA

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