Treatment with 670-nm Light Protects the Cone Photoreceptors from White Light-Induced Degeneration

  • Rizalyn S. AlbarracinEmail author
  • Krisztina Valter
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 723)


The light-induced photoreceptor damage model has been used to characterise pathological mechanisms of retinal degenerative diseases and to test potential therapeutic strategies for these conditions. This model is characterised by two fundamental processes that are common to many retinal degenerative conditions; photoreceptor cell death and loss of retinal function. In the present study, we explored the effects of 670-nm light, on cone structure and function using the light damage model in rat retina. Irradiation with 670-nm light has been shown to be beneficial in treating various disease conditions, both in humans and animal models. Sprague-Dawley rats were exposed to bright white light (BL) for 24 h. Animals were divided into three groups (n = 8/group). Each group received 670-nm light treatment at 9 J/cm2 using an LED array 1× daily for 5 days prior to light exposure (pre-conditioned), immediately after cessation of BL for 5 days (post-conditioned) or 1 day prior to BL, then 2× daily during and immediately after BL (mid-conditioned). Assessment of retinal function (ERG), structure (histology), changes in protein expression (immunohistochemistry) and gene expression (RT-qPCR) were evaluated 7 days after light exposure. Damaging effects of white light on the photoreceptor population, function and structure were ameliorated by 670-nm light in the treated groups compared to the non-treated animals (p < 0.05). Present results suggest that treatment with 670-nm light may provide long-term stability of the retina against white light-induced degeneration.


Retinal degeneration 670-nm light treatment Light damage Photoreceptor cell death Near-infrared treatment Photobiomodulation Cones 



This work was supported by the Australian Research Council through the ARC Centre of Excellence in Vision Science (CE0561903).


  1. Chrysostomou V, Stone J, Valter K (2009) Life history of cones in the rhodopsin-mutant P23H-3 rat: evidence of long-term survival. Invest Ophthalmol Vis Sci 50:2407–2416PubMedCrossRefGoogle Scholar
  2. Li Y, Tao W, Luo L et al (2010) CNTF Induces Regeneration of Cone Outer Segments in a Rat Model of Retinal Degeneration. PLoS ONE 5:e9495PubMedCrossRefGoogle Scholar
  3. Liang HL, Whelan HT, Eells JT et al (2006) Photobiomodulation partially rescues visual cortical neurons from cyanide-induced apoptosis. Neuroscience 139:639–649PubMedCrossRefGoogle Scholar
  4. Natoli R, Zhu Y, Valter K et al (2010) Gene and noncoding RNA regulation underlying photoreceptor protection: microarray study of dietary antioxidant saffron and photobiomodulation in rat retina. Mol Vis 16:1801–1822PubMedGoogle Scholar
  5. Noell WK, Walker VS, Kang BS et al (1966) Retinal damage by light in rats. Invest Ophthalmol 5:450–473PubMedGoogle Scholar
  6. Remé CE, Grimm C, Hafezi F et al (1998) Apoptotic cell death in retinal degenerations. Prog Ret Eye Res 17:443–464CrossRefGoogle Scholar
  7. Rutar M, Provis J, Valter K (2010) Brief exposure to damaging light causes focal recruitment of macrophages, and long-term destabilization of photoreceptors in the albino rat retina. Curr Eye Res 35:631–643PubMedCrossRefGoogle Scholar
  8. Valter, K, Bisti S, Gargini C et al (2005) Time course of neurotrophic factor upregulation and ­retinal protection against light-induced damage after optic nerve section. Invest Ophthalmol Vis Sci 46:1748–1754PubMedCrossRefGoogle Scholar
  9. Whelan HT, Smits RL, Buchman EV et al (2001) Effect of NASA Light-Emitting Diode Irradiation on Wound Healing. J Clin Laser Med & Surg 19:305–314CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Research School of BiologyThe Australian National UniversityCanberraAustralia
  2. 2.ARC Centre of Excellence for Vision SciencesThe Australian National UniversityCanberraAustralia

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