Abstract
Pigment epithelium-derived factor receptor (PEDF-R) is a patatin-like phospholipase domain-containing 2 (PNPLA2) protein recently identified in the retina. It has high affinity for pigment epithelium-derived factor (PEDF), a protein that acts on the neural and vascular retina. PEDF-R is a membrane-linked phospholipase and PEDF binding stimulates its enzymatic phospholipase A2 activity, which catalyzes the cleavage of fatty acids from membrane phospholipids. In this study, we mapped the epitope of an antibody for PEDF-R using recombinant PEDF-R polypeptides fragments and synthetic peptides. In addition to mapping the recognition site of the antibody on PEDF-R, we have identified peptides that specifically block its immunoreactivity and will prove to be useful tools for PEDF-R research.
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References
Barnstable CJ, Tombran-Tink J (2004) Neuroprotective and antiangiogenic actions of PEDF in the eye: molecular targets and therapeutic potential. Prog Retin Eye Res 23:561–577
Bazan NG (2005) Neuroprotectin D1 (NPD1): a DHA-derived mediator that protects brain and retina against cell injury-induced oxidative stress. Brain Pathol 15:159–166
Bazan NG, Marcheselli VL, Hu J et al (2005) Pigment epithelium-derived growth factor (PEDF) selectively up-regulates NPD1 synthesis and release through the apical side of human RPE cells in primary cultures. Invest Ophthalmol Vis Sci 46:167
Bouck N (2002) PEDF: anti-angiogenic guardian of ocular function. Trends Mol Med 8:330–334
Crawford SE, Stellmach V, Ranalli M et al (2001) Pigment epithelium-derived factor (PEDF) in neuroblastoma: a multifunctional mediator of Schwann cell antitumor activity. J Cell Sci 114:4421–4428
Garcia M, Fernandez-Garcia NI, Rivas V et al (2004) Inhibition of xenografted human melanoma growth and prevention of metastasis development by dual antiangiogenic/antitumor activities of pigment epithelium-derived factor. Cancer Res 64:5632–5642
Murakami Y, Ikeda Y, Yonemitsu Y et al (2008) Inhibition of nuclear translocation of apoptosis-inducing factor is an essential mechanism of the neuroprotective activity of pigment epithelium-derived factor in a rat model of retinal degeneration. Am J Pathol 173:1326–1338
Notari L, Baladron V, Aroca-Aguilar JD, Balko N et al (2006) Identification of a lipase-linked cell membrane receptor for pigment epithelium-derived factor. J Biol Chem 281:38022–38037
Notari L, Miller A, Martinez A et al (2005) Pigment epithelium-derived factor is a substrate for matrix metalloproteinase type 2 and type 9: implications for downregulation in hypoxia. Invest Ophthalmol Vis Sci 46:2736–2747
Subramanian P, Notario PM, Becerra SP (2010) Pigment Epithelium-derived Factor Receptor (PEDF-R): A Plasma Membrane-linked Phospholipase with PEDF Binding Affinity. Adv Exp Med Biol 664:29–37
Wang L, Schmitz V, Perez-Mediavilla A et al (2003) Suppression of angiogenesis and tumor growth by adenoviral-mediated gene transfer of pigment epithelium-derived factor. Mol Ther 8:72–79
Locatelli-Hoops S, Notari L, Becerra SP (2008) Identification of Structural Determinants on PEDF-R Responsible for Binding PEDF. ARVO Meeting Abstracts. Invest Ophthalmol Vis Sci 49:5768
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Subramanian, P., Rapp, M., Becerra, S.P. (2012). Identification of Pigment Epithelium-Derived Factor Receptor (PEDF-R) Antibody Epitopes. In: LaVail, M., Ash, J., Anderson, R., Hollyfield, J., Grimm, C. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 723. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-0631-0_102
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DOI: https://doi.org/10.1007/978-1-4614-0631-0_102
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