Identification of Pigment Epithelium-Derived Factor Receptor (PEDF-R) Antibody Epitopes

  • Preeti SubramanianEmail author
  • Matthew Rapp
  • S. Patricia Becerra
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 723)


Pigment epithelium-derived factor receptor (PEDF-R) is a patatin-like phospholipase domain-containing 2 (PNPLA2) protein recently identified in the retina. It has high affinity for pigment epithelium-derived factor (PEDF), a protein that acts on the neural and vascular retina. PEDF-R is a membrane-linked phospholipase and PEDF binding stimulates its enzymatic phospholipase A2 activity, which catalyzes the cleavage of fatty acids from membrane phospholipids. In this study, we mapped the epitope of an antibody for PEDF-R using recombinant PEDF-R polypeptides fragments and synthetic peptides. In addition to mapping the recognition site of the antibody on PEDF-R, we have identified peptides that specifically block its immunoreactivity and will prove to be useful tools for PEDF-R research.


PEDF-R antibody Epitope PEDF Phospholipase A2 PNPLA2 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Preeti Subramanian
    • 1
    Email author
  • Matthew Rapp
    • 2
  • S. Patricia Becerra
    • 1
  1. 1.National Eye Institute, National Institutes of HealthBethesdaUSA
  2. 2.University of Maryland Baltimore CountyBaltimoreUSA

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