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Identification of Pigment Epithelium-Derived Factor Receptor (PEDF-R) Antibody Epitopes

  • Preeti SubramanianEmail author
  • Matthew Rapp
  • S. Patricia Becerra
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 723)

Abstract

Pigment epithelium-derived factor receptor (PEDF-R) is a patatin-like phospholipase domain-containing 2 (PNPLA2) protein recently identified in the retina. It has high affinity for pigment epithelium-derived factor (PEDF), a protein that acts on the neural and vascular retina. PEDF-R is a membrane-linked phospholipase and PEDF binding stimulates its enzymatic phospholipase A2 activity, which catalyzes the cleavage of fatty acids from membrane phospholipids. In this study, we mapped the epitope of an antibody for PEDF-R using recombinant PEDF-R polypeptides fragments and synthetic peptides. In addition to mapping the recognition site of the antibody on PEDF-R, we have identified peptides that specifically block its immunoreactivity and will prove to be useful tools for PEDF-R research.

Keywords

PEDF-R antibody Epitope PEDF Phospholipase A2 PNPLA2 

References

  1. Barnstable CJ, Tombran-Tink J (2004) Neuroprotective and antiangiogenic actions of PEDF in the eye: molecular targets and therapeutic potential. Prog Retin Eye Res 23:561–577PubMedCrossRefGoogle Scholar
  2. Bazan NG (2005) Neuroprotectin D1 (NPD1): a DHA-derived mediator that protects brain and retina against cell injury-induced oxidative stress. Brain Pathol 15:159–166PubMedCrossRefGoogle Scholar
  3. Bazan NG, Marcheselli VL, Hu J et al (2005) Pigment epithelium-derived growth factor (PEDF) selectively up-regulates NPD1 synthesis and release through the apical side of human RPE cells in primary cultures. Invest Ophthalmol Vis Sci 46:167Google Scholar
  4. Bouck N (2002) PEDF: anti-angiogenic guardian of ocular function. Trends Mol Med 8:330–334PubMedCrossRefGoogle Scholar
  5. Crawford SE, Stellmach V, Ranalli M et al (2001) Pigment epithelium-derived factor (PEDF) in neuroblastoma: a multifunctional mediator of Schwann cell antitumor activity. J Cell Sci 114:4421–4428PubMedGoogle Scholar
  6. Garcia M, Fernandez-Garcia NI, Rivas V et al (2004) Inhibition of xenografted human melanoma growth and prevention of metastasis development by dual antiangiogenic/antitumor activities of pigment epithelium-derived factor. Cancer Res 64:5632–5642PubMedCrossRefGoogle Scholar
  7. Murakami Y, Ikeda Y, Yonemitsu Y et al (2008) Inhibition of nuclear translocation of apoptosis-inducing factor is an essential mechanism of the neuroprotective activity of pigment epithelium-derived factor in a rat model of retinal degeneration. Am J Pathol 173:1326–1338PubMedCrossRefGoogle Scholar
  8. Notari L, Baladron V, Aroca-Aguilar JD, Balko N et al (2006) Identification of a lipase-linked cell membrane receptor for pigment epithelium-derived factor. J Biol Chem 281:38022–38037PubMedCrossRefGoogle Scholar
  9. Notari L, Miller A, Martinez A et al (2005) Pigment epithelium-derived factor is a substrate for matrix metalloproteinase type 2 and type 9: implications for downregulation in hypoxia. Invest Ophthalmol Vis Sci 46:2736–2747PubMedCrossRefGoogle Scholar
  10. Subramanian P, Notario PM, Becerra SP (2010) Pigment Epithelium-derived Factor Receptor (PEDF-R): A Plasma Membrane-linked Phospholipase with PEDF Binding Affinity. Adv Exp Med Biol 664:29–37PubMedCrossRefGoogle Scholar
  11. Wang L, Schmitz V, Perez-Mediavilla A et al (2003) Suppression of angiogenesis and tumor growth by adenoviral-mediated gene transfer of pigment epithelium-derived factor. Mol Ther 8:72–79PubMedCrossRefGoogle Scholar
  12. Locatelli-Hoops S, Notari L, Becerra SP (2008) Identification of Structural Determinants on PEDF-R Responsible for Binding PEDF. ARVO Meeting Abstracts. Invest Ophthalmol Vis Sci 49:5768Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Preeti Subramanian
    • 1
    Email author
  • Matthew Rapp
    • 2
  • S. Patricia Becerra
    • 1
  1. 1.National Eye Institute, National Institutes of HealthBethesdaUSA
  2. 2.University of Maryland Baltimore CountyBaltimoreUSA

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