Finding a Novel Threadlike Structure on the Intra-abdominal Organ Surface of Small Pigs by Using In Vivo Trypan Blue Staining
Recently K.S. Soh and his colleagues in Seoul National University discovered novel threadlike structures named primo-vessels in organ surfaces, blood vessels, lymph vessels, and spinal cord in rabbits, rats, and mice. In this work, we tried to investigate whether the same structures can be found on the internal organ surface of small pigs. After being anesthetized with phenobarbital sodium and the abdomen opened, Trypan blue was applied to visualize the primo-vessels and primo-nodes on the internal organ surface of seven small pigs. Threadlike, movable, elastic structures could be observed on five small pigs. Morphological study on the obtained samples revealed that there are lots of rod-shaped nucleuses longitudinally along the structure. The structure is similar to the structures observed by K.S. Soh and his team in rat, rabbit, and mice. Further study is needed to investigate the function of these structures.
KeywordsTrypan Blue Alcian Blue Alcian Blue Trypan Blue Solution Feulgen Reaction
This work was supported by a 973 project grant from the Ministry of Science and Technology of China (2010CB530507).
- 1.Cho SJ, Kim BS, Park YS (2004) Threadlike structures in the aorta and coronary artery of swine. J Int Soc Life Info Sci 22:609–611Google Scholar
- 3.Lee BC, Park ES, Nam TJ, Johng HM, Baik KY, Sung B et al (2004) Bonghan ducts on the surface of rat internal organs. J Int Soc Life Info Sci 22:455–459Google Scholar
- 4.Lee KJ, Kim SY, Jung TE, Jin D, Kim DH, Kin HW (2004) Unique duct system and the corpusclelike structures found on the surface of the liver. J Int Soc Life Info Sci 22:460–462Google Scholar
- 5.Kim BH (1963) On the Kyungrak system. J Acad Med Sci 10:1–41Google Scholar
- 6.Fujiwara S, Yu SB (1967) “Bonghan theory”morphological studies. Igakuno Aumi 60:567–577Google Scholar
- 10.Zhang WB, Zhuang FY, Tian YY, Li H (2001) A simulating study of biophysical features along meridians on a gel model. J Biomed Eng 18(3):357–361Google Scholar