Visualization of the Primo Vascular System by Using Trypan Blue in the Subarachnoid Space of Rats
One of the most recognized theories for the acupuncture meridian has been the concept that acupuncture effects propagate through the nervous system. Beyond this general concept in Western medicine, there was one revolutionary idea for the acupuncture meridian, named “Bonghan theory.” The Bonghan theory insisted that acupuncture effects transmit not via the nerve system but via specific channels, named Bonghan ducts (primo vessels, PVs). Bonghan theory insisted that the PVs also existed in the nerve system. In order to visualize the nerve primo vessels (NPVs) under arachnoid mater of rat, Trypan blue (TB) was intrathecally injected into the spinal cord of rats. TB worked to visualize NPVs as effectively as in previous works. The NPVs were investigated by using light microscopic and transmission electron microscopic (TEM) images. Those images of the NPVs showed that they had regular patterned rod-shaped nuclei and that many microparticles existed as in other NPVs inside rabbit brain ventricles and central canals. The new structure, the NPVs, under the arachnoid mater of the spinal cord of rats could encourage one to reconsider that there may be other potential channels in the nerve system corresponding to acupuncture meridian.
KeywordsSpinal Cord Trypan Blue Central Canal Intrathecal Injection Trypan Blue
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) in 2010 (No. 2010-0025289) and by the Korean Pharmacopuncture Foundation Grant funded by the Korean Pharmacopuncture Institute (KPI-2010-003).
- 1.Kim BH (1965) Kyungrak system. J Acad Sci DPR Korea 168:1–38Google Scholar
- 6.Dai JX, Lee BC, An P et al. In vivo in situ staining of the primo vascular system in ventricles and subarachnoid space of brain by injecting Trypan blue into the lateral ventricle submitted to Anatomical RecordGoogle Scholar
- 8.Lee BC, Soh KS (2009) A Novel Model for Meridian: Bonghan Systems Combined with Fascia (Bonghan-Fascia Model). Proceedings, Fascia Research II: Basic Science and Implications for Conventional and Complementary Healthcare. Amsterdam, Elsevier, p 144Google Scholar