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Structure and Mechanism of Eukaryotic Type IIA Topoisomerases

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DNA Topoisomerases and Cancer

Part of the book series: Cancer Drug Discovery and Development ((CDD&D))

Abstract

Type II topoisomerases maintain DNA superhelicity and disentangle chromosomal segments to support cell division and viability. These activities are carried out by an ATP-dependent, duplex strand-passage mechanism that relies on the transient and reversible enzyme-mediated cleavage of DNA. A wealth of biochemical and structural data on Top2 (topo II), the eukaryotic member of this topoisomerase family, as well as complementary studies on its bacterial counterparts (DNA gyrase and topo IV), has helped explain many essential features of this complex catalytic cycle.

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Acknowledgements

The authors thank Karl Drlica for critical reading and helpful comments on this chapter. This work was supported by the NCI (CA077373, to JMB) and NIGMS (GM033944, to NO).

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Authors and Affiliations

  1. Department of Molecular and Cell Biology, QB3 Institute, University of California at Berkeley, 374D Stanley Hall #3220, Berkeley, CA, 94720, USA

    James M. Berger

  2. Departments of Biochemistry and Medicine (Hematology/Oncology), School of Medicine, Vanderbilt University, 654 Robinson Research Building, Nashville, TN, 37232-0146, USA

    Neil Osheroff

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Correspondence to James M. Berger or Neil Osheroff .

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  1. Laboratory of Molecular Pharmacology, National Institutes of Health Laboratory of Molecular Pharmacology, Bethesda, Maryland, USA

    Yves Pommier

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Berger, J.M., Osheroff, N. (2012). Structure and Mechanism of Eukaryotic Type IIA Topoisomerases. In: Pommier, Y. (eds) DNA Topoisomerases and Cancer. Cancer Drug Discovery and Development. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-0323-4_4

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