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Investigative Methodology for Chronobiology

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Biological Rhythms and Medicine

Part of the book series: Topics in Environmental Physiology and Medicine ((TEPHY))

Abstract

Progress in the developing science of chronobiology has closely paralleled the emergence of new and improved research methodologies. Methodological requirements for biological rhythm study have been reviewed previously (Halberg et al. 1972, 1977; Reinberg 1971, 1974; Smolensky et al. 1974). This chapter outlines a minimum set of conditions and procedures necessary for conducting sound chronobiologic investigations. The recommendations which are put forward should be regarded as proposals and/or suggestions rather than rules or criteria for judging the quality of experimentation. It is obvious that each study dictates a specific methodology depending upon, among other things, the goals of the investigation and the state of knowledge. The contents of this chapter provide necessary information for designing chronobiologic research protocols and for minimizing the occurrence of those types of mistakes typically experienced in earlier chronobiologic investigations.

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References

  • Apfelbaum, M, Reinberg, A, Nillus, P, and Halberg, F (1969) Rythmes circadiens de l’alternance veille-sommeil pendant l’isolement souterrain de sept jeunes femmes. Presse Médicale 77: 879–882.

    PubMed  CAS  Google Scholar 

  • Aschoff, J (1960) Exogenous and endogenous components in circadian rhythms. Cold Spring Harbor Symp on Quant Biol 25: 11–28.

    Article  CAS  Google Scholar 

  • Aschoff, J, Klotter, K, and Weyer, R (1965) Circadian vocabulary. In: Aschoff, J, ed, Circadian Clocks. Amsterdam: North-Holland Publ. Co., pp. 10–19.

    Google Scholar 

  • Aschoff, J, Fatranska, M, Giedke, H, Doerr, P, Stamm, D, and Wisser, H (1971) Human circadian rhythms in continuous darkness: entrainment by social cues. Science 171: 213–215.

    Article  PubMed  CAS  Google Scholar 

  • Aschoff, J (1981) Annual rhythms in man. In: Aschoff, J, ed, Handbook of Behavioral Neurobiology, Vol. 4. London: Plenum Press, pp. 475–487.

    Google Scholar 

  • Barcal, R, Soya, J, Krizanovska, M, Levy, J, and Matousek, J (1968) Genetic background of circadian rhythms. Nature 220: 1128–1131.

    Article  PubMed  CAS  Google Scholar 

  • Batschelet, E, Hillman, D, Smolensky, M, and Halberg, F (1973) Angular-linear correlation coefficient for rhythmometry and circannually changing human birth rates at different geographic latitudes. Int J Chronobiol 1: 183–202.

    PubMed  CAS  Google Scholar 

  • Bicakova-Rocher, A, Gorceix, A, and Reinberg, A (1981) Possible circadian rhythm alterations in certain healthy human adults (effects of a placebo). In: Reinberg, A, Vieux, N, and Andlauer, P, eds, Night and Shiftwork: Biological and Social Aspects. Oxford: Perga-mon Press, pp. 297–310.

    Google Scholar 

  • Boissin, J, and Assenmacher, I (1971) Entrainment of the adrenal cortical rhythm and of the locomotor activity rhythm by ahemeral photo-periods in the quail. J Interdiscipl Cycle Res 2: 437–443.

    Article  Google Scholar 

  • Brody, AW, Vander, HJ, O’Halloran, PF, Connolly, JJ, and Schwertley, FW (1964) Correlation, normal standards, and interdependence in test of ventilatory strength and mechanics. Ann Rev Res Dis 89: 214–235.

    CAS  Google Scholar 

  • Brown, FA (1965) Propensity for lunar periodicity in hamsters and its significance for biological clocks theories. Proc Soc Exper Biol Med 120: 792–797.

    Google Scholar 

  • van Cauter, E (1974) Methods for the analysis of multifrequential biological time series. J Interdiscipl Cycle Res 5: 131–148.

    Article  Google Scholar 

  • Cornelissen, G, Halberg, F, Stebbings, J, Halberg, E, Carandente, F, and Hsi, B (1980) Chronobiometry with pocket calculators and computer systems. La Ricerca in Clinica e in Laboratorio 10 (2).

    Google Scholar 

  • Czeisler, CA, Richardson, GS, Zimmerman, JC, Moore, MC, and Weitzman, ED (1981) Entrainment of human circadian rhythms by light—dark cycles: a reassessment. Photochem Photobiol 34: 239–247.

    PubMed  CAS  Google Scholar 

  • Del Pozo, F, Jimenez, J, and De Feudis, F (1979) Method for the acquisition and analysis of chronopharmacological data. In: Reinberg, A, and Halberg F, eds, Chronopharmacology. Oxford: Pergamon Press, pp. 185–192.

    Google Scholar 

  • De Prins, J (1975) Limitation des modeles mathématiques. Implications en ce qui concerne les transferts de méthodes. In: Delattre, P, ed, Elaboration et justification des modèles. Applications en biologie. Paris: Malone, pp. 243–253.

    Google Scholar 

  • De Prins, J, Waetens, E, and Cornelissen, G (1976) Influence de la forme du signal sur la détermination de l’acrophase. Bulletin du Groupe d’Etudes des Rythmes Biologiques 8: 123–129.

    Google Scholar 

  • De Prins, J, Cornelissen, G, and Halberg, F (1977) Harmonic interpolation on equispaced series covering integral period of anticipated circadian rhythm in adriamycin tolerance. Chronobiologia 4: 173 (abstract).

    Google Scholar 

  • De Prins, J, Cornelissen, G, Hillman, D, Halberg, F, and Van Dijck. (in press) Harmonic interpolation yields paraphases and orthophases for biologic rhythms. Laboratoire des Etalons de Fréquence. Université Libre de Bruxelles Tervuren, Belgique pbl. No. 80, 1978, and Proc Int Conf Int Soc for Chronobiology, Pavia, 1978.

    Google Scholar 

  • Descovich, GC, Montalbetti, N, Kuhl, JFW, Himondos, S, and Halberg, F (1974) Age and catecholamine rhythms. Chronobiologia 1: 163–171.

    PubMed  CAS  Google Scholar 

  • Dupont, W, Bourgeois, P, Reinberg, A, and Vaillant, R (1979) Circannual and circadian rhythms in the concentration of corticosterone in the plasma of the edible frog (Rana esculenta l.). J Endocrinol 80: 117–125.

    Article  PubMed  CAS  Google Scholar 

  • Duruisseau, JP (1965) Seasonal variation of PBI in healthy Montrealers. J Clin Endocrinol Me-tab 25: 1513–1515.

    Article  CAS  Google Scholar 

  • Gaultier, C, Reinberg, A, and Girard, F (1977) Circadian rhythms in lung resistance and dynamic lung compliance of healthy children. Effects of two bronchodilators. Respiration Physiology 31: 169–182.

    Article  PubMed  CAS  Google Scholar 

  • Gervais, P, and Reinberg, A (1976) The clinical significance of chronobiological methods for allergic asthma. In: McGovern, JP, Smolensky, M, and Reinberg, A, eds, Chrono-biology in Allergy and Immunology. Springfield, Ill., Charles C Thomas, pp. 64–78.

    Google Scholar 

  • Ghata, J, Reinberg, A, Lagoguey, M, and Touitou, Y (1977) Human circadian rhythms documented in May—June from three groups of young healthy males living respectively in Paris, Colombo and Sydney. Chronobiologia 4: 181–190.

    PubMed  CAS  Google Scholar 

  • Guillemant, J, Eurin, J, Guillemant, S, and Reinberg, A (1979) Circadian and other rhythms in corticoadrenal cyclic nucleotides (cAMP—cGMP) of intact and hypophysectomized male, mature rats. Chronobiologia 6: 101–105.

    Google Scholar 

  • Gupta, BD, and Deka, AC (1975) Application of chronobiology to radio-therapy of tumor of oral cavity. Chronobiologia, 2 (Suppl. 1): 25 (abstract).

    Google Scholar 

  • Halberg, F (1973) Laboratory techniques and rhythmometry. In: Mills, JN, ed, Biological Aspects of Circadian Rhythms. London: Plenum, pp. 1–26.

    Chapter  Google Scholar 

  • Halberg, F, and Nelson, W (1978) Chronobiologic optimization of aging. In: Smith, HV, and Capobianco, S, eds, Aging and Biological Rhythms. London: Plenum, pp. 5–55.

    Google Scholar 

  • Halberg, F, and Simpson, H (1967) Circadian acrophase of human 17-hydroxycorticosteroid excretion referred to midsleep rather than midnight. Human Biol 39: 405–413.

    PubMed  CAS  Google Scholar 

  • Halberg, F, Halberg, E, Barnum, CP, and Bittner, JJ (1959) Physiologic 24-hour periodicity in human beings and mice, the lighting regi-men and daily routine. In: Withrow, RB, ed, Photoperiodism and Related Phenomena in Plants and Animals. Washington, D.C.: A.A.A.S. 55: 803–878.

    Google Scholar 

  • Halberg, F, Engeli, M, Hamburger, C, and Hillman, D (1965) Spectral resolution of low-frequency, small amplitude rhythms in excreted ketosteroid; probable androgen-induced circaseptan desynchronization. Acta Endocrinol Suppl 103: 5–54.

    Google Scholar 

  • Halberg, F, Tong, YL, and Johnson EA (1967) Circadian system phase: an aspect of temporal morphology; procedure and illustrative examples. In: von Mayersbach, H, ed, The Cellular Aspects of Biorhythms. Berlin: Springer, pp. 20–48.

    Google Scholar 

  • Halberg, F, Johnson, EA, Nelson, W, Runge, W, and Sothern, R (1972) Autorhythmometry. Procedures for physiologic self-measurements and their analysis. Physiol Teacher 1: 1–11.

    Google Scholar 

  • Halberg, F, Carandente, F, Cornelissen, G, and Katinas, GS (1977) Glossary of chrono-biology. Chronobiologia, 4 (suppl. 1): 1–189.

    PubMed  Google Scholar 

  • Halberg, F, Reinberg, A, and Lagoguey, M (1983) Circannual rhythms in man. Int J Chronobiol (in press).

    Google Scholar 

  • Haus, E, and Halberg, F (1970) Circannual rhythm in level and timing of serum corticosterone in standardized inbred mature C-mice. Environ Res 3: 81–106.

    Article  PubMed  CAS  Google Scholar 

  • Klein, KE, and Wegmann, HM (1979) Circadian rhythms of human performance and resistance: operational aspects. In: AGARD lecture series no. 105, Sleep, Wakefulness and Circadian Rhythm. Neuilly S/Seine 92200 France: AGARD-NATO publications, pp. 2. 10–2. 12.

    Google Scholar 

  • Marotte, H (1979) Etudes des rhythmes biologiques par analyse de variance: recherche expérimentale du rhythme circadien de la régulation thermique chez l’homme. Thèse Dr. ès-Sciences. Université Claude Bernard, Lyon France, Février, p. 348.

    Google Scholar 

  • Martin, W (1981) Estimation of parameters of circadian rhythms, if measurements cannot be performed around the clock. The case of a band limited signal plus noise. In: Reinberg, A, Vieux, N, and Andlauer, P, eds, Night and Shiftwork: Biological and Social Aspects. Oxford: Pergamon Press, pp. 37–44.

    Google Scholar 

  • von Mayersbach, H (1978) Die Zeitstruktur des Organismus. Arzneimittelforschung/Drug Research 28 (11): 1824–1836.

    Google Scholar 

  • McGovern, MP, Smolensky, MH, and Reinberg, A (1977) Circadian and circamensual rhythmicity in cutaneous reactivity to histamine and allergenic extracts. In: McGovern, JP, Smolensky, MH, and Reinberg, A, eds, Chronobiology in Allergy and Immunology. Springfield, Ill.: Charles C Thomas, pp. 79–116.

    Google Scholar 

  • Nelson, W, Tong, YK, Jueng—Kuen, L, and Halberg, F (1979) Methods for cosinor-rhythmometry. Chronobiologia 6: 305–323.

    CAS  Google Scholar 

  • Prevost, EJ, Smolensky, MH, Reinberg, A, and McGovern, JP (1980) Circadian rhythm of respiratory distress in asthmatic, bronchitic and emphysemic patients. In: Smolensky, MH, Reinberg, A, and McGovern, JP, eds, Recent Advances in the Chronobiology of Allergy and Immunology. Oxford: Pergamon Press, Ltd., pp. 237–250.

    Google Scholar 

  • Procacci, P, Buzzelli, G, Passeri, I, Sassi, R, Viegelin, MR, and Zoppi, M (1972) Studies on the cutaneous pricking pain threshold in man, circadian and circatrigintan changes. Res Clin Stud Headache 3: 260–276.

    Google Scholar 

  • Reinberg, A (1971) Methodologic considerations for human chronobiology. J Interdiscpl Cycle Res 2: 1–15.

    Article  Google Scholar 

  • Reinberg, A (1974) Aspects of circannual rhythms in man. In: Pengelley, ET, ed, Circannual Clocks. New York: Academic Press, pp. 423–505.

    Google Scholar 

  • Reinberg, A, Vieux, N, Ghata, J, Chaumont, A-J, and Laporte, A (1979) Consideration of the circadian amplitude in relation to the ability to phase shift circadian rhythms of shift workers. Chronobiologia 6 (Suppl. 1): 57–63.

    Google Scholar 

  • Reinberg, A, Zagulla—Mally, Z, Ghata, J, and Halberg, F (1969) Circadian reactivity rhythms in human skin to house dust, penicillin and histamine. J Allergy 44: 292–306.

    CAS  Google Scholar 

  • Reinberg, A, Gervais, P, Halberg, Francine, and Halberg, Franz (1971) Trisentinel monitoring of air pollution by autorhythmometry of peak expiratory flow. Proc 2nd Int Clean Air Cong. New York: Academic Press, pp. 217–220.

    Google Scholar 

  • Reinberg, A, Lagoguey, M, Chauffournier, JM, and Cesselin, F (1975) Circannual and circadian rhythms in plasma testosterone in five healthy young Parisian males. Acta Endocrinol 80: 732–743.

    PubMed  CAS  Google Scholar 

  • Reinberg, A, Lagoguey, M, Cesselin, F, Touitou, Y, Legrand, JC, Delasalle, A, Antreassian, J, and Lagoguey, A (1978) Circadian and circannual rhythms in plasma hormones and other variables of five healthy young human males. Acta Endocrinol 88: 417427.

    Google Scholar 

  • Reinberg, A, Vieux, N, Laporte, A, Andlauer, P, and Smolensky, M (1979) Chronobiological field studies of oil refinery shift workers. Chronobiologia 6 (Suppl 1): 1–119.

    Google Scholar 

  • Rutenfranz, J (1978) Schichtarbeit und biologische Rhythmik. Arzneimittel Forschung/Drug Research 28 (11): 1867–1872.

    CAS  Google Scholar 

  • Scheving, LE, Sohal, GS, Enna, D, and Pauly, JE (1973) The persistence of a circadian rhythm in histamine response in guinea pigs maintained under continuous illumination. Anat Rec 175: 1–7.

    Article  PubMed  CAS  Google Scholar 

  • Simpson, HW, and Bohlen, JG (1973) Latitude and the human circadian system. In: Mills, JN, ed., Biological Aspects of Circadian Rhythms. London: Plenum Press, pp. 85–118

    Chapter  Google Scholar 

  • Simpson, H, and Halberg, E (1974) Menstrual changes of the circadian temperature rhythm in women. In: Ferin, M, Halberg, F, Richart, RM, and Van de Wiele, RL, eds, Biorhythms and Human Reproduction. New York: John Wiley, pp. 549–556.

    Google Scholar 

  • Smolensky, MH (1976) Chronobiologic aspects of cutaneous reactivity, an example of endogenous rhythmicity and implications upon the evaluation of environmental quality. In: Gervais, P, ed, Asthme, Allergie Respiratoire et Environnement Socio-Ecologique. Le Mont-Dore meeting, mai 1975. Imprimeca 19200 Ussel, pp. 41–60.

    Google Scholar 

  • Smolensky, MH, Reinberg, A, Lee, RE, and McGovern, JP (1974) Secondary rhythms related to hormonal changes in the menstrual cycle. In: Ferin, M, Halberg, F, Richart, RM, and Van de Wiele, R, eds, Biorhythms and Human Reproduction. New York: John Wiley, pp. 287–306.

    Google Scholar 

  • Smolensky, MH, Halberg, F, Harter, J, Hsi, B, and Nelson, W (1978) Higher corticosterone values at a fixed single timepoint in serum from mice `trained’ by prior handling. Chronobiologia 5: 1–13.

    PubMed  CAS  Google Scholar 

  • Smolensky, MH, Halberg, F, Pitts, GC, and Nelson, W (1980a). The chronopharmacology of methylprednisolone: clinical implications of animal studies with special emphasis upon the moderation of growth inhibition by timing to circadian rhythms. In: Smolensky, MH, Reinberg, A, and McGovern, JP, eds, Recent Advances in the Chronobiology of Allergy and Immunology. Oxford: Pergamon Press, pp. 137–171.

    Google Scholar 

  • Smolensky, MH, Reinberg, A, and McGovern, JP, eds. (1980b) Recent Advances in the Chronobiology of Allergy and Immunology. Oxford: Pergamon Press.

    Google Scholar 

  • Sollberger, A (1970) Problems in the statistical analysis of short periodic time series. J Interdiscipl Cycle Res. 1: 49–88.

    Article  Google Scholar 

  • Tammeling, GJ, De Vries, K, and Kruyt, EW (1977) The circadian pattern of the bronchial reactivity to histamine in healthy subjects and in patients with obstructive lung disease. In: McGovern, JP, Smolensky, MH, and Reinberg, A, eds, Chronobiology in Allergy and Immunology. Springfield: Charles C Thomas, pp. 139–149.

    Google Scholar 

  • Weitzman, ED, Fukushima, D, Nogeire, C, Roffwarg, H, Gallager, TF, and Hellman, L (1971) Twenty-four hour pattern of the episodic secretion of cortisol in normal subjects. J Clin Endocrinol Metab 33: 14–22.

    Article  PubMed  CAS  Google Scholar 

  • Weyer, RA (1965) A mathematical model for circadian rhythms. In: Aschoff, J, ed, Circadian Clocks. Amsterdam: North-Holland Pub. Co., pp. 47–63.

    Google Scholar 

  • Weyer, RA (1979) The Circadian System of Man: Results of Experiments under Temporal Isolation. New York: Springer-Verlag.

    Google Scholar 

  • Winfree, AT (1980) The Geometry of Biological Time. Biomathematics, Vol. 8. New York: Springer-Verlag.

    Google Scholar 

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Authors
  1. Alain Reinberg
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  2. Michael H. Smolensky
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Reinberg, A., Smolensky, M.H. (1983). Investigative Methodology for Chronobiology. In: Biological Rhythms and Medicine. Topics in Environmental Physiology and Medicine. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-9496-9_2

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  • DOI: https://doi.org/10.1007/978-1-4613-9496-9_2

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4613-9498-3

  • Online ISBN: 978-1-4613-9496-9

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