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Fluorescence Polarization to Evaluate the Fluidity of Natural and Reconstituted Membranes

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Fluorescence Studies on Biological Membranes

Part of the book series: Subcellular Biochemistry ((SCBI,volume 13))

Abstract

Fluorescence polarization measurements assess movement of membrane constituents under near-normal conditions, as those membranes are challenged with toxic and/or nontoxic ions. Data interpretation of fluorescence polarization measurements are contingent upon an understanding of the interactions between the plasma membrane and other cellular constituents. Therefore, a brief summarization of a number of known interactions between the plasma membrane and other cellular components forms the basis of evaluations of membrane fluidity using fluorescence polarization.

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  1. Department of Pharmacology and Toxicology, College of Pharmacy, University of Texas, Austin, Texas, 78712, USA

    Elsie M. B. Sorensen

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  1. Elsie M. B. Sorensen
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  1. University of Antwerp, Antwerp, Belgium

    H. J. Hilderson

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Sorensen, E.M.B. (1988). Fluorescence Polarization to Evaluate the Fluidity of Natural and Reconstituted Membranes. In: Hilderson, H.J. (eds) Fluorescence Studies on Biological Membranes. Subcellular Biochemistry, vol 13. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-9359-7_5

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