Abstract
It has become clear that the preimplantation embryo possesses a number of rapidly developing and sometimes unique systems for transmembrane transport. For example, mouse embryos possess amino acid transport systems that change from one set to a different set, found only in embryos, over the course of only a few days’ development (1); excitable calcium channels are found in abundance in the early mouse and hamster embryo, but disappear completely by the 16-cell stage (2–4); and Na+ transport into the rabbit blastocoel is completely altered between the 5th and 7th day of development, with the development of amiloride and furosemide sensitivity (5, 6).
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References
Van Winkle LJ. Amino acid transport in developing animal oocytes and early conceptuses. Biochim Biophys Acta 1988;947:173–208.
Eusebi F, Colonna R, Mangia F. Development of membrane excitability in mammalian oocytes and early embryos. Gamete Res 1983;7:39–47.
Mitani S. The reduction of calcium current associated with early differentiation of the murine embryo. J Physiol 1985;363:71–86.
Yoshida S. Action potentials dependent on monovalent cations in developing mouse embryos. Dev Biol 1985;110:200–6.
Powers RD, Borland RM, Biggers JD. Amiloride-sensitive rheogenic Na+ transport in rabbit blastocyst. Nature (London) 1977;270:603–4.
Benos DJ, Biggers JD. Sodium and chloride co-transport by preimplantation rabbit blastocysts. J Physiol 1983;342:23–33.
Cohen BJ, Lechene C. Na,K pump: cellular role and regulation in non-excitable cells. Biol Cell 1989;66:191–5.
Montrose MH, Murer H. Kinetics of Na+/H+ exchange. In: Grinstein S, ed. Na+/H+ exchange. Boca Raton, FL: CRC Press, 1988:57–75.
Aronson PS, Nee J, Suhm MA. Modifier role of internal H+ in activating the Na+/H+ exchanger in renal microvillus membrane vesicles. Nature (London) 1982;299:161–3.
Sardet C, Counillon L, Franchi A, Pouyssegur J. Growth factors induce phosphorylation of the Na+/H+ antiporter, a glycoprotein of 110 kD. Science 1990;247:723–6.
Kopito RR, Lee BS, Simmons DM, Lindsey AE, Morgans CW, Schneider K. Regulation of intracellular pH by a neuronal homolog of the erythrocyte anion exchanger. Cell 1989;59:927–37.
Olsnes S, Tonnessen TI, Sandvig K. pH-regulated anion antiport in nucleated mammalian cells. J Cell Biol 1986;102:967–71.
Vaughan-Jones RD. Regulation of intracellular pH in cardiac muscle. In: Bock G, Marsh J, eds. Proton passage across cell membranes. CIBA Foundation Symposium 139, Feb. 9–11, 1988. London: Wiley and Sons, 1988:23–46.
Moolenaar WH, Tsien RY, van der Saag PT, de Laat SW. Na+/H+ exchange and cytoplasmic pH in the action of growth factors in human fibroblasts. Nature (London) 1983;304:645–8.
Moolenaar WH, Tertoolen LGJ, de Laat SW. Phorbol esters and diacylglycerol mimic growth factors in raising cytoplasmic pH. Nature (London) 1984;312: 371–3.
Ganz MB, Boyarsky G, Sterzel RB, Boron WF. Arginine vasopressin enhances pHi regulation in the presence of HCO3 - by stimulating three acid-base transport systems. Nature (London) 1989;337:648–51.
Biermann AJ, Tertoolen LGJ, de Laat SW, Moolenaar WH. The Na+/H+ exchanger is constitutively activated in P19 embryonal carcinoma cells, but not in a differentiated derivative. J Biol Chem 1987;262:9621–8.
Perona R, Serrano R. Increased pH and tumorigenicity of fibroblasts expressing a yeast proton pump. Nature (London) 1988;334:438–40.
Schwartz MA, Both G, Lechene C. Effect of cell spreading on cytoplasmic pH in normal and transformed fibroblasts. Proc Natl Acad Sci USA 1989; 86:4525–9.
Epel D. The role of Na+/H+ exchange and intracellular pH changes in fertilization. In: Grinstein S, ed. Na+/H+ exchange. Boca Raton, FL: CRC Press, 1988:57–75.
Boron WF, de Weer P. Intracellular pH transients in squid giant axons caused by CO2, NH3, and metabolic inhibitors. J Gen Physiol 1976;67: 91–112.
Roos A, Boron WF. Intracellular pH. Physiol Rev 1981;61:296–434.
Molecular Probes product information for BCECF-AM and BCECF. Apr. 1991 edition. Molecular Probes, Inc., Eugene, OR.
Bright GR, Fisher GW, Rogowska J, Taylor DL. Fluorescence ratio imaging microscopy: temporal and spatial measurements of cytoplasmic pH. J Cell Biol 1987;104:1019–33.
Baltz JM, Biggers JD, Lechene C. Apparent absence of Na+/H+ antiport activity in the two-cell mouse embryo. Dev Biol 1990;138:421–9.
Baltz JM, Biggers JD, Lechene C. Relief from alkaline-load in two-cell stage mouse embryos by bicarbonate/chloride exchange. J Biol Chem 1991;266: 17212–7.
Thomas RC. The role of bicarbonate, chloride and sodium ions in the regulation of intracellular pH in snail neurones. J Physiol 1977;273:317–38.
Boyarsky G, Ganz MB, Sterzel RB, Boron WF. pH regulation in single glomerular mesangial cells, I. Acid extrusion in absence and presence of HCO3 -. Am J Physiol 1988;255:C844–56.
Boron WF, Boulpaep EL. Intracellular pH regulation in the renal proximal tubule of the salamander: Na+/H+ exchange. J Gen Physiol 1983;81:29–52.
Aickin CC. Movement of acid equivalents across the mammalian smooth muscle membrane. In: Bock G, Marsh J, eds. Proton passage across cell membranes. CIBA Foundation Symposium 139, Feb. 9–11, 1988. London: Wiley and Sons, 1988:23–46.
Bidani A, Brown SES, Heming TA, Gurich R, Dubose TD. Cytoplasmic pH in pulmonary macrophages: recovery from acid-loads is Na+ independent and NEM sensitive. Am J Physiol 1989;257:C65–76.
Lubman RL, Danto SI, Crandall ED. Evidence for active H+ secretion by rat alveolar epithelial cells. Am J Physiol 1989;257:L438–45.
Baltz JM, Biggers JD, Lechene C. Two-cell stage mouse embryos appear to lack mechanisms for alleviating intracellular acid-loads. J Biol Chem 1991; 266:6052–7.
Muallem S, Burnham C, Blissard D, Berglindh T, Sachs G. Electrolyte transport across the basolateral membrane of the parietal cells. J Biol Chem 1985;260:6641–53.
Kurtz I, Golchini K. Na+-independent Cl--HCO3 - exchange in Madin-Darby canine kidney cells: role in intracellular pH regulation. J Biol Chem 1987;262:4516–20.
Reinertsen KV, Tonnessen TI, Jacobsen J, Sandvig K. Role of chloride/bicarbonate antiport in the control of cytoplasmic pH: cell line differences in activity and regulation of antiport. J Biol Chem 1988;263:11117–25.
Manejwala FM, Cragoe EJ, Schultz RM. Blastocoel expansion in the pre-implantation mouse embryo: role of extracellular sodium and chloride and possible apical routes of their entry. Dev Biol 1989;133:210–20.
Borland RM, Hazra S, Biggers JD, Lechene C. The elemental composition of the environments of the gametes and preimplantation embryo during the initiation of pregnancy. Biol Reprod 1977;16:147–57.
Maas DHA, Storey BT, Mastroianni L. Hydrogen ion and carbon dioxide content of the oviductal fluid of the rhesus monkey (Macaca mulatto). Fertil Steril 1977;28:981–5.
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© 1993 Springer-Verlag New York, Inc.
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Baltz, J.M., Biggers, J.D., Lechene, C. (1993). Intracellular pH Regulation by the Preimplantation Embryo. In: Bavister, B.D. (eds) Preimplantation Embryo Development. Serono Symposia, USA Norwell, Massachusetts. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-9317-7_8
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DOI: https://doi.org/10.1007/978-1-4613-9317-7_8
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