Oxytocin Receptors in Myometrium: Preliminary Evidence for a Receptor Switch Mechanism

  • Vladimir Pliška
  • Hildegard Kohlhauf Albertin
  • Denis J. Crankshaw
Part of the Serono Symposia USA Norwell, Massachusetts book series (SERONOSYMP)

Abstract

The phasic contractile activity of mammalian uterus is controlled by several hormones, among them oxytocin. Oxytocin (OT) increases contractile activity in a rapid, concentration-dependent manner. This effect is of importance under certain physiological circumstances, most obviously during delivery, and a precise control of the onset and cessation of OT action is a necessary feature of this regulatory system. How can this control be achieved?

Keywords

Hydrolysis Magnesium Filtration Lithium Progesterone 

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References

  1. 1.
    Soloff MS, Fields MJ. Changes in uterine oxytocin receptor concentrations throughout the estrous cycle of the cow. Biol Reprod 1989;40:283–7.PubMedCrossRefGoogle Scholar
  2. 2.
    Fuchs A-R, Fuchs F, Husslein P, Soloff MS, Fernström MJ. Oxytocin receptors and human parturition: a dual role for oxytocin in the initiation of labor. Science 1982;215:1396–8.PubMedCrossRefGoogle Scholar
  3. 3.
    Pliška V. Pharmacological versus binding analysis of receptor systems: how do they interplay? Myometrial cell receptors for oxytocin as a paradigm. Experientia 1991;47:216–21.PubMedCrossRefGoogle Scholar
  4. 4.
    Soloff MS, Swartz TL. Characterization of a proposed oxytocin receptor in the uterus of the rat and sow. J Biol Chem 1974;249:1376–81.PubMedGoogle Scholar
  5. 5.
    Crankshaw DJ, Branda LA, Matlib MA, Daniel EE. Localization of the oxytocin receptor in the plasma membrane of rat myometrium. Eur J Biochem 1978;86:481–6.PubMedCrossRefGoogle Scholar
  6. 6.
    Pliška V, Heiniger J, Müller-Lhotsky A, Pliska P, Ekberg B. Binding of oxytocin to uterine cells in vitro. Occurence of several binding site populations and reidentification of oxytocin receptors. J Biol Chem 1986;261: 16984–9.PubMedGoogle Scholar
  7. 7.
    Crankshaw DJ, Gaspar V, Pliška V. Multiple [3H]-oxytocin binding sites in rat myometrial plasma membranes. J Recept Res 1990;10:269–85.PubMedGoogle Scholar
  8. 8.
    Pliška V, Kohlhauf Albertin H. Effect of magnesium on the binding of oxytocin to sheep myometrial cells. Biochem J 1991;277:97–101.PubMedGoogle Scholar
  9. 9.
    Burton AC. The properties of steady state compared to those of equilibrium as shown in characteristic biological behaviour. J Cell Comp Physiol 1939; 14:327–49.CrossRefGoogle Scholar
  10. 10.
    Marc S, Leiber D, Harbon S. Carbachol and oxytocin stimulate the generation of inositol phosphates in the guinea pig myometrium. FEBS Lett 1986; 201:9–14.PubMedCrossRefGoogle Scholar
  11. 11.
    Schrey MP, Read AM, Steer PJ. Oxytocin and vasopressin stimulate inositol phosphate production in human gestational myometrium and decidua cells. Biosci Rep 1986;6:613–9.PubMedCrossRefGoogle Scholar
  12. 12.
    Ruzycky AL, Crankshaw DJ. Role of inositol phospholipid hydrolysis in the initiation of agonist-induced contractions of rat uterus: effect of domination by 17β-estradiol and progesterone. Can J Physiol Pharmacol 1988;66:10–7.PubMedCrossRefGoogle Scholar
  13. 13.
    Pliška V. Pharmacological approaches to the identification and classification of myometrial oxytocin receptors. J Receptor Res 1988;8:245–59.Google Scholar
  14. 14.
    Furchgott RF, Bursztyn P. Comparison of dissociation constants and of relative efficacies of selected agonists acting on parasympathetic receptors. Ann NY Acad Sci 1967;144:882–99.CrossRefGoogle Scholar
  15. 15.
    Pliška V, Heiniger J. Structural requirements of the oxytocin receptor in rat uterus. Free-Wilson analysis in a series of competitive oxytocin inhibitors. Int J Pept Protein Res 1988;31:520–36.PubMedGoogle Scholar
  16. 16.
    Berridge MJ, Downes CP, Hanley MR. Lithium amplifies agonist-dependent phosphatidylinositol responses in brain and salivary glands. Biochem J 1982; 206:587–95.PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1993

Authors and Affiliations

  • Vladimir Pliška
  • Hildegard Kohlhauf Albertin
  • Denis J. Crankshaw

There are no affiliations available

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