Measurement of Retina and Optic Nerve Oxidative Metabolism in Vivo via Dual Wavelength Reflection Spectrophotometry of Cytochrome a, a3
The energy metabolism of the retina and optic nerve is maintained in a steady state, utilizing and producing free energy. This energy is mainly derived from glycolysis, the tricarboxylic acid (i.e., Kreb’s) cycle, and the mitochondrial oxidative phosphorylation pathway (i.e., respiratory chain). Each step is carefully controlled, ranging from blood flow autoregula-tion (which adjusts the supply of glucose and oxygen to the cell) to the rate of electron flow in the oxidative phosphorylation pathway, which is coupled to the production of adenosine triphosphate (ATP). Various techniques have been employed to monitor retinal and optic nerve energy metabolism in vivo and in vitro.
KeywordsIschemia Mercury Chrome Lactate Respiration
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