The Regulation of Blast Stem Cell Self-Renewal in Myeloblastic Leukemia

  • E. A. McCulloch
Conference paper
Part of the Experimental Hematology Today—1988 book series (HEMATOLOGY, volume 1988)


The blast cells of Acute Myeloblastic Leukemia are considered as a novel lineage, composed of segments of normal differentiation programs assembled abnormally, reflecting abnormal gene expression. AML blast cells can be grown in culture; the earliest blast progenitors have been shown to have the stem cell capacity of self-renewal. This paper considers certain aspects of self-renewal regulation. First, based on drug survival curves measured in two culture techniques, the suggestion is made that the balance between self-renewal and differentiation is regulated by specific genes. Second evidence is presented that blast proliferative patterns are affected by the growth factors IL-3, GM-CSF, G-CSF and CSF-1. The most consistent pattern is seen with CSF-1, where cells show decreased self-renewal when exposed to CSF-1 added to cultures or to the products of the CSF-1 gene as detected by Northern analysis. Finally, the importance of self-renewal is emphasized. It is proposed that drugs might be classified by their effect on this crucial cell function; further, that the chemosensitivity of self-renewal is an attribute contributing to clinical outcome.


Blast Cell Cytosine Arabinoside Acute Myeloblastic Leukemia Acute Myeloblastic Leukemia Patient Hemopoietic Growth Factor 
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© Springer Science+Business Media New York 1989

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  • E. A. McCulloch

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