Gene Transfer by Electroporation

  • A. Keating
  • F. Toneguzzo
Conference paper
Part of the Experimental Hematology Today—1988 book series (HEMATOLOGY, volume 1988)


The development of efficient means of gene transfer into hematopoietic progenitor cells has permitted investigators to analyze stem cell relationships [1] and has increased the feasibility of gene therapy protocols [2]. Recent studies suggest that electroporation (electric-field-mediated DNA transfer) may be a particularly attractive technique for the latter purpose.


K562 Cell Long Terminal Repeat Rous Sarcoma Virus Human Hematopoietic Stem Cell Gene Therapy Protocol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Keller G, Paige C, Gilboa E, Wagner EF (1985) Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent hematopoietic precursors. Nature 318: 149.PubMedCrossRefGoogle Scholar
  2. 2.
    Dick JE, Magli MC, Phillips RA, Bernstein A (1986) Genetic manipulation of hematopoietic stem cells. Trends in Genetics 2: 165–170.CrossRefGoogle Scholar
  3. 3.
    Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH (1982) Gene transfer into mouse myeloma cells by electroporation in high electric fields EMBO J 7: 841.Google Scholar
  4. 4.
    Toneguzzo F, Hayday A, Keating A (1986) Electric field mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells. Mol Cell Biol 6 (2): 703–706.PubMedGoogle Scholar
  5. 5.
    Toneguzzo F, Keating A, Glynn S, McDonald K (1988) Electric field-mediated gene transfer: characterization of DNA transfer and patterns of integration in lymphoid cells. Nucleic Acids Res 16 (12): 5515–5533.PubMedCrossRefGoogle Scholar
  6. 6.
    Toneguzzo F, Keating A (1986) Stable expression of selectable genes introduced into human hematopoietic stem cells by electric field mediated DNA transfer. Proc Natl Acad Sci USA 83: 3496–3499.PubMedCrossRefGoogle Scholar
  7. 7.
    Takahashi M, Keating A, Singer JW (1985) A functional defect in irradiated adherent layers from chronic myelogenous leukemia long-term marrow cultures. Exp Hematol 13: 926–931.PubMedGoogle Scholar
  8. 8.
    Gorman CM, Rigby PWJ, Lane DP (1985) Negative regulation of viral enhancers in undifferentiated embryonic stem-cells. Cell 42: 519–526.PubMedCrossRefGoogle Scholar
  9. 9.
    Lozzio CB, Lozzio BB (1975) Human chronic myelogenous leukemia cell line with positive Philadelphia chromosome. Blood 45: 321–334.PubMedGoogle Scholar
  10. 10.
    Keating A, Just-Mitchell K, Toor P, Klein M, Sodek J (1986) Passaged human marrow stromal cells: a unique cell population. Exp Hematol 14 (6): 426.Google Scholar
  11. 11.
    Keating A, Powell J, Takahashi M, Singer JW (1984) The generation of human long-term marrow cultures from marrow depleted of la (HLA-DR) positive cells. Blood 64 (6): 1159–1162.PubMedGoogle Scholar
  12. 12.
    Gorman CM, Moffat LF, Howard BH (1982) Recombinant genomes which express chloramphenicol acetyl transferase in mammalian cells. Mol Cell Biol 2: 1044–1051.PubMedGoogle Scholar
  13. 13.
    Gunning T, Leavitt J, Muscat G, Ng S-Y, Kedes L (1987) A human ß-actin expression vector system directs high level accumulation of antisense transcripts. Proc Natl Acad Sci USA 84: 4831–4851.PubMedCrossRefGoogle Scholar
  14. 14.
    Mosthaf L, Pawlita M, Gruss P (1985) A viral enhancer element specifically active in human haematopoietic cells. Nature 315: 597–600.PubMedCrossRefGoogle Scholar
  15. 15.
    Xian-Jun F, Keating A, de Villiers J, Sherman M (submitted) Tissue specific activity of heterologous viral promotors in primary rat hepatocytes and Hep G2 cells.Google Scholar
  16. 16.
    Norgard MV (1981) Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNASE. Anal Biochem 113: 34–42.PubMedCrossRefGoogle Scholar
  17. 17.
    Neumann JR, Morency CA, Russian KO (1987) A novel rapid assay for chloramphenicol acetyltransferase gene expression. Biotechniques 5: 444–447.Google Scholar
  18. 18.
    Bertling W, Hunger-Bertling K, Cline MJ (1987) Intranuclear uptake and persistance of biologically active DNA after electroporation of mammalian cells. J Biochem Biophys Methods 14: 223–232.PubMedCrossRefGoogle Scholar
  19. 19.
    Boggs SS, Gregg RG, Borenstein N, Smithies O (1986) Efficient transformation and frequent single-site, single copy insertion of DNA can be obtained in mouse erythroleukemia cells transformed by electroporation. Exp Hematol 14: 988–994.PubMedGoogle Scholar
  20. 20.
    Jastreboff MM, Ito E, Bertino JR, Narayan R (1987) Use of electroporation for high-molecular-weight DNA-mediated gene transfer. Exp Cell Res 171 (2): 513–517.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • A. Keating
  • F. Toneguzzo

There are no affiliations available

Personalised recommendations