Applications of a New Membrane Print Technique in Biotechnology

  • P. M. O’Neill
  • M. B. Singh
  • R. B. Knox
Conference paper


In exploring the biotechnology of pollen, nitrocellulose membrane is used extensively for electro-blotting of proteins from polyacrylamide gels, e.g. dot-blotting (Hawkes et al., 1982). The proteins bind electrostatically to the membrane surface and can then be stained with various probes, e.g. general protein stains, chromophore substrates for enzymes, lectin or lectin-like molecules for glyco-conjugates and monoclonal or monospecific polyclonal antibodies for antigens. Nitrocellulose membrane has been utilized to make pollen prints or micro-dot blots of surface proteins of pollen of oilseed rape, Brassica campestris and ryegrass Lolium perenne.


Pollen Quality Pollen Wall Pollen Protein Amido Black Printing Time 
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Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • P. M. O’Neill
  • M. B. Singh
  • R. B. Knox
    • 1
  1. 1.Plant Cell Biology Research Centre, School of BotanyUniversity of MelbourneParkvilleAustralia

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