Advertisement

Applications of a New Membrane Print Technique in Biotechnology

  • P. M. O’Neill
  • M. B. Singh
  • R. B. Knox
Conference paper

Abstract

In exploring the biotechnology of pollen, nitrocellulose membrane is used extensively for electro-blotting of proteins from polyacrylamide gels, e.g. dot-blotting (Hawkes et al., 1982). The proteins bind electrostatically to the membrane surface and can then be stained with various probes, e.g. general protein stains, chromophore substrates for enzymes, lectin or lectin-like molecules for glyco-conjugates and monoclonal or monospecific polyclonal antibodies for antigens. Nitrocellulose membrane has been utilized to make pollen prints or micro-dot blots of surface proteins of pollen of oilseed rape, Brassica campestris and ryegrass Lolium perenne.

Keywords

Pollen Quality Pollen Wall Pollen Protein Amido Black Printing Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Hawkes R, Niday E, Gordon J (1982) A dot-immunobinding assay for monoclonal and other antibodies. Analyt Biochem 119: 142–147.PubMedCrossRefGoogle Scholar
  2. Heslop-Harrison J, Heslop-Harrison Y, Knox RB, Howlett B (1973) Pollen wall proteins: ‘Gametophytic and sporophytic’ fractions in the pollen walls of the Malvaceae. Ann Bot 37: 403–412.Google Scholar
  3. Heslop-Harrison J, Knox RB, Heslop-Harrison Y, Mattsson O (1975) Pollen wall proteins: emission and role in incompatibility responses. In: Duckett JG, Racey PA (eds) The Biology of the Male Gamete. Academic Press, New York, pp 189–202.Google Scholar
  4. Howlett BJ, Vithanage HIMV, Knox RB (1981) Immunofluorescence localization of two water soluble glycoproteins, including the major allerqen of ryegrass, Lolium perenne. Histochem J 13: 461–480.PubMedCrossRefGoogle Scholar
  5. Kerhoas C, Knox RB, Dumas C (1983) Specificity of the callose response in stigmas of Brassica Ann Bot 52: 597–602.Google Scholar
  6. Roberts IN, Stead AD, Ockendon DJ, Dickinson HG (1980) Pollenstigma interactions in Brassica oleracea Theor Appl Genet 58: 241–246.CrossRefGoogle Scholar
  7. Singh MB, Knox RB (1984) Quantitative cytochemistry of β-galactosidase in normal (Gal) and enzyme deficient (gal) pollen of Brassica campestris: an application of the indigogenic method. Histochem J 16: 1273–1296.PubMedCrossRefGoogle Scholar
  8. Singh MB, Knox RB (1985) Immunofluorescence applications in plant cells. In: Robards AW (ed) Botanical Microscopy 1985. Oxford University Press, Oxford (in press).Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • P. M. O’Neill
  • M. B. Singh
  • R. B. Knox
    • 1
  1. 1.Plant Cell Biology Research Centre, School of BotanyUniversity of MelbourneParkvilleAustralia

Personalised recommendations