Abstract
In contrast to plant tissue culture procedures which promote callus proliferation, cell suspensions and plant propagation, the culture of flower and inflorescence primordia can be designed to favor normal growth and differentiation. The technique has revealed details of the nutritional requirements for development and the biochemical features of organ maturation and differentiation (Tepfer et al., 1962; Raman and Greyson, 1978; Bilderback, 1971). Prominent in these investigations has been the use of the plant growth regulators frequently associated with sexual differentiation (Galun et al. 1962).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Bilderback DE (1971) The effects of amino acids upon the development of excised floral buds of Aquilegia. Amer J Bot 58: 203–208
Flavell R, Mathias R (1984) Prospects for transforming monocot crop plants. Nature 307: 108–109
Galun E, Jung Y, Lang A (1962) Culture and sex modification of male cucumber in vitro Nature 194: 596–598
Pareddy DR, Greyson RI, Walden DB (1985) In vitro germination of pollen from cultured tassels. Maize Gen Coop Newslett 59: 73–74
Polowick PL, Raman K, Greyson RI (1981) In vitro liquid culture of corn tassels. Maize Gen Coop Newslett 55: 116
Polowick PL, Greyson RI (1982) Anther development, meiosis and pollen formation in Zea tassels cultured in defined liquid medium. Plant Sci Lett 26: 139–45
Polowick PL, Greyson RI (1985) Microsporogenesis and gametophyte maturation in cultured tassels of Zea mays L. Can J Bot (in press)
Raman K, Greyson RI (1978) Further observations on the differential sensitivites to plant growth regulators by cultured’ single’ and ‘double’ flower buds of Nigella damascena (Rananculaceae). Amer J Bot 65: 180–191
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Springer-Verlag New York Inc.
About this paper
Cite this paper
Greyson, R.I., Pareddy, D.R., Bommineni, V.R., Walden, D.B. (1986). The Use of In Vitro Methods in the Production of Pollen. In: Mulcahy, D.L., Mulcahy, G.B., Ottaviano, E. (eds) Biotechnology and Ecology of Pollen. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8622-3_23
Download citation
DOI: https://doi.org/10.1007/978-1-4613-8622-3_23
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-8624-7
Online ISBN: 978-1-4613-8622-3
eBook Packages: Springer Book Archive