Cross-reactivity of Anti-lymphocyte Monoclonal Antibodies on Human Skin Components
Monoclonal antibodies have become extraordinary specific tools for identifying cell subpopulations such as, for example, lymphocytes. In utilizing them in such a manner, it has become clear that due to their specificity for a given epitope, they can also give apparent loss of specificity when reacting with one epitope shared by two different unrelated antigens. We have prepared monoclonal antibodies against human lymphoid cells with the aim of selecting antibodies specific for antigens defining lymphocyte subpopulations. In the course of the selection process, we screened hybridoma supernatants on normal human skin as a non-lymphoid organ. We found unexpected and interesting staining patterns (1) which led us to generalize these control screenings and to select monoclonal antibodies recognizing most human skin cells. The 159 monoclonal antibodies of the panel for T cell studies were screened on human skin sections in the same manner; 21 of them were found to react on skin components. The possible biological significances of these cross-reactivities will be discussed.
KeywordsNormal Human Skin Basal Keratinocytes Human Lymphoid Cell Skin Component Antilymphocyte Antibody
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