Epitopic Groups of CD1 Molecules

  • Laurence Boumsell
  • Martine Amiot
  • Brigitte Raynal
  • Véronique Gay-Bellile
  • Bernard Caillou
  • Alain Bernard

Abstract

The first cluster of differentiation, CD1 [Thy, gp45, 12], defined by the First International Workshop on Human Leucocyte Differentiation Antigens (1) included antibodies NA1/34, T6, D47, and M241. The antibodies reacted with thymocytes, but not peripheral T cells, with some T-ALL cells from both children and adults, and with some T-lymphoid cell lines. Antibodies D47 (2) and M241 (3) particularly were dissociated from NA1/34 (4) and T6 (5) in, respectively, T-ALL cells from adults and T-ALL cells from children. Biochemically all of the CD1 antibodies recognize a structure which is similar to that of human HLA-class I molecules with approximately 45,000-M r heavy chains attached to β2-microglobulin.

Keywords

Lymphoma Leukemia Polypeptide Paraffin Myeloma 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bernard, A., L. Boumsell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. 1984. Leucocyte typing. Springer-Verlag, Berlin, Heidelberg.Google Scholar
  2. 2.
    Boumsell, L., C. Gelin, H. Coppin, P. Caudan, J. Kalil, D. Pham B. Raynal, and A. Bernard. 1984. Diversity of class I molecules heavy and light chains on human thymus cells. In: Leucocyte typing, A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. Springer-Verlag, Berlin, Heidelberg.Google Scholar
  3. 3.
    Knowles, R.K., and W.F. Bodmer. 1982. A monoclonal antibody recognizing a human TL-like antigen. Eur. J. Immunol. 12: 676.PubMedCrossRefGoogle Scholar
  4. 4.
    McMichael, A.J., J.R. Pilch, G. Galfre, D.Y. Mason, J.W. Fabre, and C. Milstein. 1979. A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur. J. Immunol. 9: 205.PubMedCrossRefGoogle Scholar
  5. 5.
    Reinherz, E.L., P.C. Kung, G. Goldstein, R.L. Levey, and S.F. Schlossman. 1980. Discrete stages of intrathymic differentiation analysis of normal thymocytes and leukemic lymphoblasts of T lineage. Proc. Natl. Acad. Sci. U.S.A. 77: 1588.PubMedCrossRefGoogle Scholar
  6. 6.
    Bernaben, C., M. Van de Rijn, P.G. Lerch, and C.P. Terhorst. 1984. β2Microglobulin from serum associates with MHC class I antigens on the surface of cultured cells. Nature 308: 642.CrossRefGoogle Scholar
  7. 7.
    Kefford, R.F., F. Calabi, I.M. Fearnely, O.R. Burrone, and C. Milstein. 1984. Serum ß2-microglobulin binds to a T-cell differentiation antigen and increases its expression. Nature 308: 641.PubMedCrossRefGoogle Scholar
  8. 8.
    Ziegler, A., and C. Milstein. 1979. A small polypeptide different from 02microglobulin associated with a human cell surface antigen. Nature 279: 243.PubMedCrossRefGoogle Scholar
  9. 9.
    Knowles, R.W. 1984. Biochemical analysis of human thymus leukemia-like antigens. In: Leucocyte typing, A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. Springer-Verlag, Berlin, Heidelberg.Google Scholar
  10. 10.
    Takezaki, S., S.L. Morrison, C.L. Berger, G. Goldstein, A.C. Chu, and R.L. Edelson. 1982. Biochemical characterization of a differentiation antigen shared by human epidermal langerhans cells and cortical thymocytes. J. Clin. Immunol. 2: 1288.Google Scholar
  11. 11.
    Kanitakis, J., D. Schmitt, A. Bernard, L. Boumsell, and J. Thivolet. 1983. Anti-D47: a monoclonal antibody reacting with the secretory cells of human eccrine sweat glands. Brit. J. Dermatol. 109: 509.CrossRefGoogle Scholar
  12. 12.
    Boumsell, L., and A. Bernard. 1980. High efficiency of Biozzi’s high responder mouse strain in the generation of antibody secreting hybridomas. J. Immunol. Methods 138: 225.CrossRefGoogle Scholar
  13. 13.
    Boumsell, L., M. Amiot, B. Raynal, V. Gay-Bellile, and A. Bernard. Manuscript in preparation.Google Scholar
  14. 14.
    Teillaud, J.L., D. Crevat, P. Chardon, J. Kalil, C. Goujet-Zali, G. Mahony, M. Vaiman, M. Fellous, and D. Pious. 1982. Monoclonal antibodies as a tool for phylogenetic studies of major histocompatibility antigens and B2 microglobulin. Im m un ogen. 15: 377.Google Scholar
  15. 15.
    Bernard, A., C. Gelin, B. Raynal, D. Pham, C. Goose, and L. Boumsell. 1982. Phenomenon of human T-cells rosetting with sheep erythrocytes analyzed with monoclonal antibodies. “Modulation” of a partially hidden epitope determining the conditions of interactions between T-cells and erythrocytes. J. Exp. Med. 155: 1317.PubMedCrossRefGoogle Scholar
  16. 16.
    Bernard, A., V. Gay-Bellile, M. Amiot, B. Caillou, P. Charbord, and L. Boumsell. 1984. A novel human leukocyte differentiation antigen: monoclonal antibody anti-D44 defines a 28 Kd molecule present on immature hematologic cells and a subpopulation of mature T-cells. J. Immunol. 132: 2338.PubMedGoogle Scholar
  17. 17.
    Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680.PubMedCrossRefGoogle Scholar
  18. 18.
    Bonner, W.M., and R.A. Laskey. 1974. A film detection method for tritium-labeled proteins and nucleic acids in polyaorylamide gels. Eur. J. Biochem. 46: 83.PubMedCrossRefGoogle Scholar
  19. 19.
    Flaherty, L., and D. Zimmerman. 1979. Surface mapping of mouse thymocytes. Proc. Natl. Acad. Sci. U.S.A. 76: 1990.PubMedCrossRefGoogle Scholar
  20. 20.
    Bourguignon, L.Y.W., R. Hyman, I Trowbridge, and S.J. Singer. 1978. Participation of histocompatibility antigens in copping of molecularly independant cell surface components by their specific antibodies. Proc. Natl. Acad. Sci. U.S.A. 75: 2406.PubMedCrossRefGoogle Scholar
  21. 21.
    Meuer, S.C., O. Acuto, R.E. Hussey, J.C. Hodgton, K.A. Fitzgerald, S.F. Schlossman, and E.L. Reinherz. 1983. Evidence for the T3 associated 90 Kd heterodimer as the T-cell antigen receptor. Nature 303: 808.PubMedCrossRefGoogle Scholar
  22. 22.
    Cotner, T., H. Mashimo, P.C. Kung, G. Goldstein, and J.L. Strominger. 1981. Human T-cell surface antigens bearing a structural relationship to HLA antigens. Proc. Natl. Acad. Sci. U.S.A. 78: 3858.PubMedCrossRefGoogle Scholar
  23. 23.
    Lemonnier, F.A., P. Le Bouteiller, B. Malissen, P. Golstein, M. Malissen, Z. Mishal, D.H. Caillol, B.R. Jordan, and F.M. Kourilsky. 1983. Transformation of murine LMTK- cells with purified HLA class I genes. J. Immunol. 130: 1432.PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • Laurence Boumsell
  • Martine Amiot
  • Brigitte Raynal
  • Véronique Gay-Bellile
  • Bernard Caillou
  • Alain Bernard

There are no affiliations available

Personalised recommendations