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Type I and Type III Restriction-Modification Enzymes

  • Robert Yuan
  • Daniel L. Hamilton
Part of the Springer Series in Molecular Biology book series (SSMOL)

Abstract

DNA methylation is the most ubiquitous form of DNA modification. It has been studied extensively in a wide variety of organisms. Although it generally is believed that DNA methylation is involved in mismatch repair in prokaryotic cells, and in the control of gene expression in eukaryotic cells, the precise nature of its biological functions remains largely unknown. One case in which the role of DNA methylation has been clearly defined is that of host-controlled restriction and modification in bacteria. This biological system consists of two highly specific enzymatic activities: an endonuclease and a DNA methylate. The endonuclease enables a given strain to both recognize and destroy foreign DNA by cutting both DNA strands at a limited number of sites. This function is defined as restriction. Kuhnlein and Arber (1972) showed that methylation at specific sequences protected the DNA from its homologous restriction endonuclease. This function is defined as modification.

Keywords

Methylase Activity Antirestriction Activity Unmethylated Site AdoMet Binding hsdR Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1984

Authors and Affiliations

  • Robert Yuan
    • 1
  • Daniel L. Hamilton
    • 1
  1. 1.NCI-Frederick Cancer Research FacilityFrederickUSA

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