Abstract
This chapter will describe relatively simple serological procedures for estimating the concentration of antibodies in a serum by agglutination. The rhizobial antigen is heat treated to inactivate the flagellar antigens and to illustrate the granular clumps characteristic of the insoluble somatic antigen reaction. The highest dilution of the serum at which positive agglutination occurs is used to define the titer.
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Key References
Vincent, J.M. 1982. Serology, pp. 235–273. In W.J. Broughton (ed.) Nitrogen Fixation, Vol. 2. Rhizobium. Clarendon Press, Oxford.
Wollum II, A.G. 1987. Serological techniques for Bradyrhizobium and Rhizobium identification. pp. 149–155. In G.H. Elkan (ed.) Symbiotic Nitrogen Fixation Technology. Marcel Dekker, Inc., New York.
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© 1994 Springer-Verlag New York, Inc.
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Somasegaran, P., Hoben, H.J. (1994). Somatic Agglutination Reactions with Pure Cultures of Rhizobia. In: Handbook for Rhizobia. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8375-8_9
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DOI: https://doi.org/10.1007/978-1-4613-8375-8_9
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-8377-2
Online ISBN: 978-1-4613-8375-8
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