Abstract
Serological methods are important for rhizobial strain identification. This chapter covers the development of antisera against several strains of rhizobia. Antigens are prepared and then injected into rabbits by three different routes. Antisera are developed for serological techniques that require soluble and insoluble somatic antigens. Injection and bleeding techniques are practiced.
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Key References
Schmidt, E.L., R.O. Bankole, and B.B. Bohlool. 1968. Fluorescent antibody approach to the study of rhizobia in soil. J. Bacteriol. 95: 1987–1997.
Vincent, J.M. 1970. A Manual for the Practical Study of Root Nodule Bacteria. IBP Handbook no. 15. Blackwell Scientific Publications, Oxford.
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© 1994 Springer-Verlag New York, Inc.
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Somasegaran, P., Hoben, H.J. (1994). Developing Antisera. In: Handbook for Rhizobia. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8375-8_8
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DOI: https://doi.org/10.1007/978-1-4613-8375-8_8
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-8377-2
Online ISBN: 978-1-4613-8375-8
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