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Counting Rhizobia by a Plant Infection Method

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Handbook for Rhizobia

Abstract

The plant infection count, also known as the most-probable-number (MPN) count, is used to determine the number of viable and infective rhizobia in the presence of other microorganisms. The trap legume selected in the MPN method must belong to the same cross-inoculation group of legumes nodulated by the rhizobia under investigation. This indirect method is commonly used to determine the quality of inoculants produced from nonsterile carrier materials. It is also used to determine the number of rhizobia in the soil.

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Key References

  • Vincent, J.M. 1970. A Manual for the Practical Study of Root Nodule Bacteria. IBP Handbook no. 15. Blackwell Scientific Publications, Oxford.

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  • Weaver, R.W., and L.R. Frederick. 1972. A new technique for the most-probable-number counts. Plant Soil 36: 219–222.

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  • Woomer, P., J. Bennett, and R. Yost. 1990. Overcoming the inflexibility of most-probable-number procedures. Agron. J. 82: 349–353.

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© 1994 Springer-Verlag New York, Inc.

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Somasegaran, P., Hoben, H.J. (1994). Counting Rhizobia by a Plant Infection Method. In: Handbook for Rhizobia. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8375-8_6

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  • DOI: https://doi.org/10.1007/978-1-4613-8375-8_6

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4613-8377-2

  • Online ISBN: 978-1-4613-8375-8

  • eBook Packages: Springer Book Archive

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