Quantifying the Growth of Rhizobia
This chapter deals with routine enumeration techniques for pure cultures of rhizobia. The total or direct count is performed using the microscope. Optical density measurements are used to estimate the number of cells in broth culture. The viable count is accomplished through plating methods. The mean generation times of a Rhizobium sp. and a Bradyrhizobium sp. in broth culture are computed.
KeywordsBroth Culture Viable Count General Microbiology Dilute Culture Wire Gauge
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