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Quantifying the Growth of Rhizobia

  • Padma Somasegaran
  • Heinz J. Hoben

Abstract

This chapter deals with routine enumeration techniques for pure cultures of rhizobia. The total or direct count is performed using the microscope. Optical density measurements are used to estimate the number of cells in broth culture. The viable count is accomplished through plating methods. The mean generation times of a Rhizobium sp. and a Bradyrhizobium sp. in broth culture are computed.

Keywords

Broth Culture Viable Count General Microbiology Dilute Culture Wire Gauge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Key References

  1. Hoben, H.J., and P. Somasegaran. 1982. Comparison of the pour, spread and drop-plate methods for the enumeration of Rhizobium spp. in inoculants made from presterilized peat. Appl. Environ. Microbiol. 44: 1246–1247.PubMedGoogle Scholar
  2. Vincent, J.M. 1970. A Manual for the Practical Study of Root Nodule Bacteria. IBP Handbook no. 15. Blackwell Scientific Publications, Oxford.Google Scholar

Copyright information

© Springer-Verlag New York, Inc. 1994

Authors and Affiliations

  • Padma Somasegaran
    • 1
  • Heinz J. Hoben
    • 1
  1. 1.University of Hawaii NifTAL ProjectPaiaUSA

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